300316 PP Fluorescence in situ Hybridization (FISH) (2010W)
Continuous assessment of course work
Labels
Block vom 7.2.2011 bis 18.2.2011, Mo-Fr jeweils 9-18 Uhr im Seminarraum Ökologie und ÜR 5.
Prüfung am 21.2.2011
Ersttermin ist Vorbesprechungstermin !
Registrierung für den Zugriff auf LV-relevante Unterlagen: www.microbial-ecology.net/education
Prüfung am 21.2.2011
Ersttermin ist Vorbesprechungstermin !
Registrierung für den Zugriff auf LV-relevante Unterlagen: www.microbial-ecology.net/education
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 09.09.2010 09:00 to Mo 27.09.2010 17:00
- Deregistration possible until Sa 30.10.2010 23:00
Details
max. 12 participants
Language: German
Lecturers
Classes (iCal) - next class is marked with N
-
Monday
07.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Tuesday
08.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Wednesday
09.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Thursday
10.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Friday
11.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Monday
14.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Tuesday
15.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Wednesday
16.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Thursday
17.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5 -
Friday
18.02.
09:00 - 18:00
Schreibraum/Seminarraum
Übungsraum 5
Information
Aims, contents and method of the course
This laboratory course will teach attendants how to specifically detect and identify prokaryotes in environmental and medical samples using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.
Assessment and permitted materials
Continuous assessment of course work. Quality of experimental work, seminar presentation; participation in the seminar discussion, qualitiy of protocol and written exam
Minimum requirements and assessment criteria
Acquisition of methods to extract DNA from environmental samples, to PCR-amplify and clone specific genes, and of FISH and fluorescence microscopy. Use of these methods on participants' own environmental samples and evaluation of the results.
Examination topics
Laboratory experiments in groups of two, introduction and discussion of the theoretical background in lectures and seminars
Reading list
Association in the course directory
B-BOE 12, B-BOE 13, B-BMG 12A, B-BMB 11A, BOE 10, BOE 11, BOE 12, BMG 10A, BMB 11A, MMI I-2,
Last modified: Sa 22.10.2022 00:30