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300317 VO Biodiversity and molecular ecology of microorganisms 1 (2012W)

2.00 ECTS (2.00 SWS), SPL 30 - Biologie

Details

Language: German

Examination dates

Lecturers

Classes (iCal) - next class is marked with N

Wednesday 03.10. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 10.10. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 17.10. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 24.10. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 31.10. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 07.11. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 14.11. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 21.11. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 28.11. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 05.12. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 12.12. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 09.01. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 16.01. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG
Wednesday 23.01. 09:00 - 11:00 Hörsaal 2 UZA 1 Biozentrum 2.249 1.OG

Information

Aims, contents and method of the course

The first part of this two-part lecture series (second part in summer semester) provides an encompassing overview on state-of-the-art molecular methods for analyzing the composition of microbial communities in complex environmental and medical samples. The lecture starts with the introduction of the bacterial species concept and parameters of microbial diversity ("species richness" and "species evenness"), followed by a detailed description of the full rRNA approach. Further techniques, which are described and critically discussed regarding their suitability for identification and quantification of microorganisms, include fluorescence in situ hybridization, quantitative real-time PCR, dot/slot blot and DNA microarray hybridization, DNA fingerprinting techniques, and novel high-throughput sequencing technologies (454/FLX, Solexa).

Assessment and permitted materials

Written Exam (1 hour), NO multiple choice!

Minimum requirements and assessment criteria

This lecture provides a detailed overview on established and cutting-edge technologies used in microbial ecology/environmental microbiology. Students are additionally made familiar with the relevant primary literature and current research results.

Examination topics

Theoretical knowledge will be acquired via lectures and original scientific literature. Demonstration of molecular techniques via animations, educational films, and technical illustrative material.
Lecture slides are available at http://www.microbial-ecology.net/education/ (registration required!)

Reading list

Madigan, Martinko, Dunlap, Clark, Brock; Biology of Microorganisms, 12th edition, Prentice Hall

Wikipedia (!Informationen immer mit der Originalliteratur abgleichen!) http://www.wikipedia.org/

Originalliteratur erhältlich über

ISI Web of Science http://isiknowledge.com

NCBI PubMed http://www.ncbi.nlm.nih.gov/sites/entrez?db=PubMed

Association in the course directory

B-BOE 5, BOE 7, B-BAN 9, B-BMG 11, B-BMB 10, B-BPB 12, B-BPF 8, B-BZO 11

Last modified: Mo 07.09.2020 15:43