300371 SE+UE Modern Methods in Molecular Biology I: Characterization and Structure Determination (2012S)

of Molecular Machines/DK Structure and Interaction of Biological Macromolecules

Continuous assessment of course work

Ort: BZB, Dr. Bohr-Gasse 9, 1030 Wien.
DK Structural Biology students only! Other DK students please contact ulrike.seifert@meduniwien.ac.at
Organisation Structure and Interaction of Biological Macromolecules

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Registration is open from Fr 03.02.2012 08:00 to Th 23.02.2012 18:00
Deregistration possible until Fr 30.03.2012 18:00

Details

max. 12 participants

Language: English

Lecturers

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

Biochemistry and Cryo Electron Microscopy
Sample Preparation and Quality Control
- How to design a purification strategy?
- Expression systems for multiple proteins
- Isolation of macromolecular complexes (Chromatography, size exclusion, centrifugation, X-linking)
- Membrane Proteins / Detergents
- Test cases / paper discussion
Electron Microscopy
- Quality control by electron microscopy
- Principles of transmission electron microscopy
- Cryo electron microscopy as a technique to solve structures of macromolecules
- Hands-on practical at the microscope
Vitrification
- Vitrification of Samples and Data collection using cryo electron microscopy
- Hands-on practical training

Assessment and permitted materials

Minimum requirements and assessment criteria

Examination topics

Reading list

Association in the course directory

PhD-MB 1, PhD-MB 2, DK-Struc

Last modified: Mo 07.09.2020 15:43