Universität Wien
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300374 PP Hands-on Cryopreparation techniques and Immunolabelling for Light and Electron Microscopy (2015W)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Lectures: Seminar Room 1, UZA 1
Workshop: EM-lab of Core Facility Cell Imaging and Ultrastructure Research.

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 4 participants
Language: English

Lecturers

Classes

First compulsory lecture October 2. 2015 12:15-14:00, Seminar Room 1, UZA1, Althanstrasse 14, 1090 Wien.
3 week block workshop inclusive November 9. 2015, until November 27. 2015, daily 0900-1700, Seminar Room 1, UZA1. Teaching language for this course is English.
Please note: Prerequisite for this advanced course is PP300674.

Information

Aims, contents and method of the course

Advanced workshop in cryopreparation methods for Ultrastructure applications. At the end of this course, participants are qualified to use the specialized machines in the EM labs for their own ultrastructure research work.

Assessment and permitted materials

Student assessment is divided into 5 parts:
(1) 20% Workshop
(2) 20% Oral assessment (end of course),
(3) 20% Final student talk (7-10 mins) followed by open discussion and questions.
(4) 20% Poster (group work)
(5) 20% Protocol of course methods and results

Please contact course leaders for further information: jacqueline.montanaro@meduniwien.ac.at and siegfried.reipert@univie.ac.at.

Minimum requirements and assessment criteria

To enable advanced BIO-stud. to understand & apply advanced cryo-electron microscopy preparation techniques and immunolabelling within their own research. Supported by lectures & demonst., this intensive, hand-on training course teaches special. techn.,such as Tokuyasu cryosectioning, high-pressure freezing-freeze substitution & acrylic resin embedding for high resol. localization of intracell. proteins & other intracell. struc. by immunogold labelling

Examination topics

Special. techn.,such as Tokuyasu cryosectioning, high-pressure freezing-freeze substitution & acrylic resin embedding, immunolabeling of proteins on semi-thin or ultra thin sections with fluorescent or colloidal gold markers. Evaluation of results with epifluorescence microscope or transmission electron microscope.

Reading list

Literature will be discussed in 1. lecture.

Association in the course directory

BBO 9, BZO 10, BOE 12, BOE 13, BZO 12, BZO 13 ,MBO 7, MMB W-2, MGE III-2, MZO 2, MEV 2, B-BZO 12, B-BZO 13, B-BOE 12, B-BOE 13, B-BPF 9, B-BPF 10

Last modified: Mo 07.09.2020 15:43