300394 VO Introduction to molecular techniques (2014W)
Labels
Details
Language: English
Examination dates
- Wednesday 03.12.2014
- Wednesday 21.01.2015
- Wednesday 28.01.2015
- Wednesday 28.01.2015
- Wednesday 04.02.2015
Lecturers
Classes
MI 10.00 - 12.00 Uhr im COSB Seminarraum, UZA 1, Biozentrum Althanstraße 14, 1090 Wien, Raum Nr. 2.022
Beginn: 08.10.2014
Information
Aims, contents and method of the course
Assessment and permitted materials
Final exam. 10:00 on two dates: 03.12.14 and 28.01.15.
Minimum requirements and assessment criteria
The goal of this course is to give an overview of the routine molecular methods used in the modern zoology and developmental biology studies. The course is aimed for the Bachelor students of all curricular directions and for the Master students specializing in non-molecular aspects of zoology.
Examination topics
Reading list
Alberts, B. et al. "Molecular biology of the cell"
Clark D.P. et al. "molecular biology"
similar textbooks
Clark D.P. et al. "molecular biology"
similar textbooks
Association in the course directory
B-WZB
Last modified: Mo 07.09.2020 15:43
The goal of this course is to give an overview of the routine molecular methods used in the modern zoology and developmental biology studies. The course is aimed for the Bachelor students of all curricular directions and for the Master students specializing in non-molecular aspects of zoology.Lecture 1: DNA chemistry “light version”
1. Structure of the DNA chain, base pairing, DNA synthesis
2. Enzymes cutting, polymerizing and modifying DNA
3. Polymerase chain reaction and its applications (RACE PCR, degenerate primer PCR etc.)
4. Vectors for cloning DNA pieces
5. Gel electrophoresisLecture 2: Genome sequencing
1. Old and new methods of genome sequencing
2. Analysis of the genome data
3. Transcriptome analysis
4. Examples of the use of the genome dataLecture 3: Gene expression analysis (Part 1)
1. Basics of transcription, splicing and translation
2. Reverse transcription
3. Quantifying the amount of transcripts
4. Localizing RNA in the tissue by in situ hybridization
5. Polyclonal and monoclonal antibodies
6. Localizing proteins in the tissue and in vitroLecture 4: Gene expression analysis (Part 2)
1. Structure of a gene
2. Regulatory elements of the gene: promoters, enhancers, insulators
3. Reporter constructs
4. Ways to search for regulatory elements: deletion mapping, phylogenetic footprinting, DNase footprinting, X-ChIP, reporter assaysLecture 5: Gene expression analysis (Part 3)
1. Testing gene regulatory elements
2. Histone and DNA modifications: role of chromatin state in the regulation of transcription
3. Ways to analyze chromatin state
4. The use of chromatin state data for prediction of the regulatory elementsLecture 6: Gene function analysis (Part 1)
1. RNA interference
2. Morpholino knockdown and mRNA rescue experiments
3. Generation of genetic knockouts and knockins
4. Conditional knockoutsLecture 7: Gene function analysis (Part 2)
1. Developmental signaling pathways and ways to analyze them
2. Generating dominant negative and constitutively active forms of proteins
3. Assaying protein-protein interactions (two-hybrid screen, BiFC)