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300394 VO Introduction to molecular techniques (2024W)
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Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Details
Language: English
Lecturers
Classes (iCal) - next class is marked with N
This lecture course consists of 7 (not 14!) lectures. If all go as planned, we will be finished by the end of November.
- N Wednesday 09.10. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 16.10. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 23.10. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 30.10. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 06.11. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 13.11. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 20.11. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 27.11. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 04.12. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 11.12. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 08.01. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 15.01. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 22.01. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
- Wednesday 29.01. 09:45 - 11:45 Seminarraum 3.1, Biologie Djerassiplatz 1, 3.124, Ebene 3
Information
Aims, contents and method of the course
Assessment and permitted materials
Oral exam. The dates will be set after the beginning of the course.
At the start of the exam, you will receive a single question in which you will have to propose an experimental strategy of solving some biological problem. You will have 20 minutes to prepare your answer. During this time, you will be allowed to use your course notes, slides or books but you will not be allowed to use the Internet. Once 20 minutes are over, you close the books, and answer your question as well as the related questions in case something in your answer is not clear.
At the start of the exam, you will receive a single question in which you will have to propose an experimental strategy of solving some biological problem. You will have 20 minutes to prepare your answer. During this time, you will be allowed to use your course notes, slides or books but you will not be allowed to use the Internet. Once 20 minutes are over, you close the books, and answer your question as well as the related questions in case something in your answer is not clear.
Minimum requirements and assessment criteria
You understand the principles underlying all the molecular techniques necessary to solve the problem in your question and explain them correctly - Grade 1You choose correct methods to solve the experimental problem in your question, but there are minor problems with explaining these methods, or you choose the wrong method but, once corrected, you can explain how the right method works - Grade 2You choose the right method but cannot explain how it works without significant help; you answer most of the additional questions correctly - Grade 3You choose the right method but cannot explain how it works without significant help; you do not answer most of the additional questions - Grade 4
Examination topics
All the molecular techniques presented during the course
Reading list
Alberts, B. et al. "Molecular biology of the cell"
Clark D.P. et al. "molecular biology"
similar textbooks
Clark D.P. et al. "molecular biology"
similar textbooks
Association in the course directory
B-WZB
Last modified: We 31.07.2024 11:06
The goal of this course is to give an overview of the routine molecular methods used in the modern zoology and developmental biology studies. The course is primarily aimed for the Bachelor students of all curricular directions and for the Master students specializing in non-molecular aspects of zoology.Lecture 1: DNA chemistry “light version”
1. Structure of the DNA chain, base pairing, DNA synthesis
2. Enzymes cutting, polymerizing and modifying DNA
3. Polymerase chain reaction and its applications (RACE PCR, degenerate primer PCR etc.)
4. Vectors for cloning DNA pieces
5. Gel electrophoresisLecture 2: Genome sequencing
1. Old and new methods of genome sequencing
2. Analysis of the genome data
3. Transcriptome analysis
4. Examples of the use of the genome dataLecture 3: Gene expression analysis (Part 1)
1. Basics of transcription, splicing and translation
2. Reverse transcription
3. Quantifying the amount of transcripts
4. Localizing RNA in the tissue by in situ hybridization
5. Polyclonal and monoclonal antibodies
6. Localizing proteins in the tissue and in vitroLecture 4: Gene expression analysis (Part 2)
1. Structure of a gene
2. Regulatory elements of the gene: promoters, enhancers, insulators
3. Reporter constructs
4. Ways to search for regulatory elements: deletion mapping, phylogenetic footprinting, DNase footprinting, X-ChIP, reporter assaysLecture 5: Gene expression analysis (Part 3)
1. Testing gene regulatory elements
2. Histone and DNA modifications: role of chromatin state in the regulation of transcription
3. Ways to analyze chromatin state
4. The use of chromatin state data for prediction of the regulatory elementsLecture 6: Gene function analysis (Part 1)
1. RNA interference
2. Morpholino knockdown and mRNA rescue experiments
3. Generation of genetic knockouts and knockins
4. Conditional knockoutsLecture 7: Gene function analysis (Part 2)
1. Developmental signaling pathways and ways to analyze them
2. Generating dominant negative and constitutively active forms of proteins
3. Assaying protein-protein interactions (two-hybrid screen, BiFC)