300402 UE Yeast Genetics and Proteomics Tools (2013S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie

Continuous assessment of course work

Vorbesprechung: 12.8. 14.00 Uhr, SR3/6.Stock, Dr.Bohrgasse 9,1030 Wien.
Termin: 26.8.-13.9.2013
ÜR/BZB, Dr.Bohrgasse 9, 1030 Wien
Weitere Infos auf der Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Registration is open from Th 21.03.2013 12:00 to Th 30.05.2013 16:00
Deregistration possible until Th 30.05.2013 16:00

Details

max. 16 participants

Language: German, English

Lecturers

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

In this laboratory course we will present methods that are required to prepare samples of sufficient quality for proteomics/MS analysis. All of the presented techniques are based on specific affinity tags and therefore we generate yeast strains by endogenous tagging of the the genes of interest. We will apply protocols for the tandem affinity purifications of whole protein complexes to study the complex composition, and also individual proteins to study their PTMs. Purified samples will be separated on SDS-PAGE and analyzed by mass spectrometry compatible gel staining techniqes. Additionally, we will demonstrate the advantages/disadvantages of a novel system to study transient protein-protein interactions called M-Track.

Assessment and permitted materials

Minimum requirements and assessment criteria

Examination topics

Reading list

Association in the course directory

MGE I-2, MGE III-2, MMB W-2, MMB I-3

Last modified: Mo 07.09.2020 15:43