300417 VO Methods in Cell Biology (2011S)
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Vorbesprechung 7. März, 2011, 14:00 Uhr
BZB/SR 3.Stock/ Dr. Bohr-Gasse 9, 1030 Wien.
Block: 2.5. bis 7.6.2011 jew. Montag und Mittwoch 13.00- 15.00 Uhr!!!
Time of lectures changed to 1:00 to 3:00 pm (instead of 2-4:00 pm)May 2:Cell culture, protein expression and purification :Primary cultures, organtypical cultures, differentiation models, eukaryotic expression systems, vector systems - Andreas BrachnerMay 4: Protein-Protein, Protein DNA interactions: In vitro binding assays, affinity (Scatchard), In situ analyses (IP), in vivo analyses, FRET, yeast two hybrid Bandshift, Footprinting, Chromatin-IP, Yeast one-hybrid Reporter Gene Assays - Andreas BrachnerMay 9: Gene Silencing techniques RNA-Interferenz; Antisense, morpholino, epigenetics - Josef GotzmannMay 11: Light Microscopy: Immunofluorescence, wide field, confocal, live cell imaging, FRAP, FRET - Josef GotzmannMay 16: Dynamics and Spatial Organisation of the Genome, Chromosome Conformation Capture techniques, GFP-tagging of chromosomes, High-resolution mapping of gene territories - Alwin KöhlerMay 18: Ananlyses of cell cycle and Tumorbiology Cell Cycle Stages (FACS, Elutriation, BrdU incorporation, proliferation marker, synchronization
EMT, telomeres / DNA damage, transgenic and knock out tumor models - Andreas Brachner, Roland FoisnerMay 23: Proteomics: Protein-identification (LC-MS/MS, mass spec, bio-informatics - Christopher GernerMay 25: Techniques in Lipid Biochemistry - Sascha MartensMay 30: X-crystallography Protein purification, Crystallisation, X-ray analyses, structure determination - Gang Dong
BZB/SR 3.Stock/ Dr. Bohr-Gasse 9, 1030 Wien.
Block: 2.5. bis 7.6.2011 jew. Montag und Mittwoch 13.00- 15.00 Uhr!!!
Time of lectures changed to 1:00 to 3:00 pm (instead of 2-4:00 pm)May 2:Cell culture, protein expression and purification :Primary cultures, organtypical cultures, differentiation models, eukaryotic expression systems, vector systems - Andreas BrachnerMay 4: Protein-Protein, Protein DNA interactions: In vitro binding assays, affinity (Scatchard), In situ analyses (IP), in vivo analyses, FRET, yeast two hybrid Bandshift, Footprinting, Chromatin-IP, Yeast one-hybrid Reporter Gene Assays - Andreas BrachnerMay 9: Gene Silencing techniques RNA-Interferenz; Antisense, morpholino, epigenetics - Josef GotzmannMay 11: Light Microscopy: Immunofluorescence, wide field, confocal, live cell imaging, FRAP, FRET - Josef GotzmannMay 16: Dynamics and Spatial Organisation of the Genome, Chromosome Conformation Capture techniques, GFP-tagging of chromosomes, High-resolution mapping of gene territories - Alwin KöhlerMay 18: Ananlyses of cell cycle and Tumorbiology Cell Cycle Stages (FACS, Elutriation, BrdU incorporation, proliferation marker, synchronization
EMT, telomeres / DNA damage, transgenic and knock out tumor models - Andreas Brachner, Roland FoisnerMay 23: Proteomics: Protein-identification (LC-MS/MS, mass spec, bio-informatics - Christopher GernerMay 25: Techniques in Lipid Biochemistry - Sascha MartensMay 30: X-crystallography Protein purification, Crystallisation, X-ray analyses, structure determination - Gang Dong
Details
Language: English
Examination dates
Lecturers
- Roland Foisner
- Andreas Brachner
- Gang Dong
- Christopher Gerner
- Josef Gotzmann
- Alwin Köhler
- Sascha Martens
Classes
Currently no class schedule is known.
Information
Aims, contents and method of the course
The lecture deals with modern state of the art methods and applications in molecular cell biological research. It is designed for students familiar with the basic theory and basic practical approaches in cell and molecular biology. The taught subjects are adapted annually to allow incorporation of new developments in the field. Currenly the topics include: modern light and electron microscopical techniques, life cell imaging, organotypical cell culture, epigenetic analyses, RNAi approaches, analyses of gene expression, cell cycle regulation, protein-protein and protein DNA interactions, DNA microarray techniques and proteomic approaches.
Assessment and permitted materials
Minimum requirements and assessment criteria
Examination topics
Reading list
Association in the course directory
MMB III-2, MMB IV-2, MMB W-2, M202, M210
Last modified: Mo 07.09.2020 15:43