300444 UE Practical Course in plantgenetic and -biotechnology (2009S)
formerly practical course in gen- and biotechnology
Continuous assessment of course work
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Ort: BZB, Dr. Bohr-Gasse 9/8.Stock, 1030 Wien.
Block: 14. - 25.9.2009
Contact: AoProf. Alisher Touraev, alisher.touraev@univie.ac.at
Informationen siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://www.students.mfpl.ac.at/
Block: 14. - 25.9.2009
Contact: AoProf. Alisher Touraev, alisher.touraev@univie.ac.at
Informationen siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://www.students.mfpl.ac.at/
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Mo 09.02.2009 10:00 to Su 22.02.2009 23:00
- Registration is open from Mo 23.02.2009 01:00 to Su 01.03.2009 23:00
- Deregistration possible until Mo 30.03.2009 23:00
Details
max. 12 participants
Language: English
Lecturers
Classes
Currently no class schedule is known.
Information
Aims, contents and method of the course
Assessment and permitted materials
Minimum requirements and assessment criteria
Examination topics
Reading list
Association in the course directory
BPF 7
Last modified: Mo 07.09.2020 15:43
a. Transient expression. Gene transfer into cultured tobacco immature pollen with reporter genes (GUS, GFP). Visualization of reporter genes (histochemical GUS assays, fluorescent light microscopy)
b. Stable transformation. Male germ line transformation (particle bombardment, in vitro maturation of microspores, in situ pollination of tobacco plants)3. Protoplast transformation
a. PEG-mediated direct gene transfer into isolated and cultured tobacco protoplasts with reporter genes (GUS, GFP). Visualization of reporter genes (histochemical, fluorimetric GUS assays; fluorescent light microscopy)4. Agrobacterium-mediated gene transfer
a. Tobacco leaf disc transformation, morphogenesis for selection.
b. Regeneration of stable transformants harbouring reporter and selectable markers (nptII). Selection of transgenic shoots on selective medium.
c. Floral dip transformation of Arabidopsis.
d. Transformation of BY2 suspension cells and selection of transformed cells on selective medium.