Universität Wien

300484 UE Practical Course in Molecular Microbiology, Microbial Ecology and Immunobiology (2013W)

Diversity and function of uncultured microbes in medical and environmental samples

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

1. Vorbesprechung am 11. November 2013 um 10:00 Uhr im Seminarraum der Division Mikrobielle Ökologie (Raum 2.309).
2. Vorbesprechung: Am 31. Januar um 11:00 Uhr im Seminarraum der Division Mikrobielle Ökologie (Raum 2.309).

Dauer: 03.02.-14.02.2014 jeweils 9.00-18.00 Uhr (Blockveranstaltung)
Ort: Seminarraum Ökologie und Übungsraum 5, UZA 1, Biozentrum Althanstraße 14.

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: English

Lecturers

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

Course goals: In this course you will learn modern methods fur studying microorganisms without cultivation, including how to identify microorganisms and visualize them directly in medical and environmental samples and how to study the activity and function of organisms in complex microbial communities.

Assessment and permitted materials

Art der Leistungskontrolle: Benotung beruht auf drei Teilleistungen: praktische Arbeit im Labor, Seminar, und abschliessende Presentation (inkl. kurzer mündl. Prüfung). Die erfolgreiche Absolvierung der LV erfordert positive Beurteilungen für alle drei Teilleistungen.

Minimum requirements and assessment criteria

Examination topics

Methods: Theoretical knowledge will be disseminated through lectures (PowerPoint Presentations), the course handout and original literature. Supervised hands-on training in independent experiments will teach you how to identify microorganisms through 16S rRNA gene/transcript analysis and fluorescence in situ hybridization techniques (FISH/ CARD-FISH). You will further learn about the use of functional genes/transcripts to identify microorganisms involved in a certain function, and how to investigate their activity with stable isotope probing incubations followed by Raman microspectroscopy. The theoretical principles of high-resolution secondary ion mass spectrometry (NanoSIMS) coupled to stable isotope incubations will also be discussed.
The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.

Reading list


Association in the course directory

MMEI I, MEC-9, MOE III-3

Last modified: Mo 07.09.2020 15:43