300484 UE Practical Course in Molecular Microbiology, Microbial Ecology and Immunobiology (2014W)
Diversity and function of uncultured microbes in medical and environmental samples
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Mo 08.09.2014 08:00 to Su 14.12.2014 18:00
- Deregistration possible until Su 14.12.2014 18:00
Details
max. 12 participants
Language: English
Lecturers
Classes
1. Vorbesprechungstermin: 19. November 2014, 10:00 Uhr im Seminarraum der Division Mikrobielle Ökologie (Raum 2.309),
2. Vorbesprechungstermin: 16. Januar 2015, 13:00 Uhr im Seminarraum der Division Mikrobielle Ökologie (Raum 2.309),
Information
Aims, contents and method of the course
Course goals: In this course you will learn modern methods fur studying microorganisms without cultivation, including how to identify microorganisms and visualize them directly in medical and environmental samples and how to study the activity and function of organisms in complex microbial communities.
Assessment and permitted materials
Art der Leistungskontrolle: Benotung beruht auf drei Teilleistungen: praktische Arbeit im Labor, Seminar, und abschliessende Presentation (inkl. kurzer mündl. Prüfung). Die erfolgreiche Absolvierung der LV erfordert positive Beurteilungen für alle drei Teilleistungen.
Minimum requirements and assessment criteria
Examination topics
Methods: Theoretical knowledge will be disseminated through lectures (PowerPoint Presentations), the course handout and original literature. Supervised hands-on training in independent experiments will teach you how to identify microorganisms through 16S rRNA gene/transcript analysis and fluorescence in situ hybridization techniques (FISH/ CARD-FISH). You will further learn about the use of functional genes/transcripts to identify microorganisms involved in a certain function, and how to investigate their activity with stable isotope probing incubations followed by Raman microspectroscopy. The theoretical principles of high-resolution secondary ion mass spectrometry (NanoSIMS) coupled to stable isotope incubations will also be discussed.
The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.
The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.
Reading list
Association in the course directory
MMEI I, MEC-9, MOE III-3
Last modified: Mo 07.09.2020 15:43