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300484 UE Practical Course in Molecular Microbiology, Microbial Ecology and Immunobiology (2016W)
Diversity and function of uncultured microbes in medical and environmental samples
Continuous assessment of course work
max. 12 participants
BlockLV vom 30.01.2017 bis 10.02.2017
1. Meeting: 7th November 2016, 9:00 am in the seminar room of the Division for Microbial Ecology (room 2.309, UZA1).
2. Meeting: 20th January 2017, 9:00 am in the seminar room of the Division for Microbial Ecology (room 2.309, UZA1).
Assessment and permitted materials
Art der Leistungskontrolle: Benotung beruht auf drei Teilleistungen: praktische Arbeit im Labor, Beteiligung und Präsentation im Seminar, und schriftliches Protokoll. Die erfolgreiche Absolvierung der LV erfordert positive Beurteilungen für alle drei Teilleistungen.
Minimum requirements and assessment criteria
Active participation in lab experiments and seminar (incl. presentation), as well as written protocol
Methods: Theoretical knowledge will be disseminated through lectures (PowerPoint Presentations), the course handout and original literature. Supervised hands-on training in independent experiments will teach you how to identify microorganisms through 16S rRNA gene/transcript analysis and fluorescence in situ hybridization techniques (FISH/ CARD-FISH). You will further learn about the use of functional genes/transcripts to identify microorganisms involved in a certain function, and how to investigate their activity with stable isotope probing incubations followed by Raman microspectroscopy. The theoretical principles of high-resolution secondary ion mass spectrometry (NanoSIMS) coupled to stable isotope incubations will also be discussed.
The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.
MMEI I, MEC-9
Last modified: Fr 31.08.2018 08:56