300484 UE Practical Course in Molecular Microbiology, Microbial Ecology and Immunobiology (2017W)
Diversity and function of uncultured microbes in medical and environmental samples
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 07.09.2017 08:50 to Mo 11.12.2017 18:00
- Deregistration possible until Mo 11.12.2017 18:00
Details
max. 12 participants
Language: English
Lecturers
Classes
Vorbesprechung am 6.11.2017, 10:00 Uhr im Seminar Raum DoME, 2.309, UZA I.
BlockLV vom 05.02.2018 bis 16.02.2018
Information
Aims, contents and method of the course
Course goals: In this course you will learn modern methods fur studying microorganisms without cultivation, including how to identify microorganisms and visualize them directly in medical and environmental samples and how to study the activity and function of organisms in complex microbial communities.Places in the course will be assigned based on the set points, the requirements of the different master programs and waiting time.
Assessment and permitted materials
Evaluation will have three parts: 1) practical work in the lab, 2) seminars, and 3) written report. Participants will need to pass all three evaluations to pass the course.
Minimum requirements and assessment criteria
Active participation in lab experiments and seminar (incl. presentation), as well as written protocol
Examination topics
Methods: Theoretical knowledge will be disseminated through lectures (PowerPoint Presentations), the course handout and original literature. Supervised hands-on training in independent experiments will teach you how to identify microorganisms through 16S rRNA gene/transcript analysis and fluorescence in situ hybridization techniques (FISH/ CARD-FISH). You will further learn about the use of functional genes/transcripts to identify microorganisms involved in a certain function, and how to investigate their activity with stable isotope probing incubations followed by Raman microspectroscopy. The theoretical principles of high-resolution secondary ion mass spectrometry (NanoSIMS) coupled to stable isotope incubations will also be discussed.
The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.
The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.
Reading list
Association in the course directory
MMEI I, MEC-9
Last modified: Mo 07.09.2020 15:43