300517 SE+UE Theory and Application of Confocal Microscopy (2016W)
(parallel courses)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from We 07.09.2016 08:00 to Th 22.09.2016 18:00
- Deregistration possible until Mo 31.10.2016 18:00
Details
max. 16 participants
Language: German
Lecturers
Classes
Introduction on 9.11.2016, 5-6pm, conference room Ecology.
Course will be held as a block from 14th - 17th November 2016, always 9 am - 6 pm.Information
Aims, contents and method of the course
After rapid development, the Confocal Laser Scanning Microscope (CLSM) became the most important imaging tool in both organismic and molecular biology. In this course, we present the theoretical background of CLSM, its application in structure analysis, in measurement and quantification of physiological parameters like membrane potential, ion concentrations or enzyme activities as well as current special techniques. In a hands-on-training, we will apply the following techniques: localisation and identification of structures with organelle specific fluorescent dyes and GFP, 3D-reconstruction and animation, 4D analysis, ratio imaging, spectral dye separation. Participants are encouraged to bring their own samples. Positive examination of this lab course is a prerequisite for independent work on the CLSM of the Cell Imaging and Ultrastructure Research Unit.
Assessment and permitted materials
Active participation in the lab course, written evaluation of the knowledge aquired in the lectures, protocol
Minimum requirements and assessment criteria
The participants know the theoretical background, the possibilities and the limitations of confocal laser scanning microscopy. They are able to work with a modern CLSM and they are familiar with techniques for sample preparation and the evaluation of results.
Examination topics
Lectures on the theoretical background of CLSM, Hands-on-training with provided samples, detailed investigation of a sample selected by the student.
Reading list
see above (literature_german)
Association in the course directory
B-WZB, WZB,
Last modified: Mo 07.09.2020 15:43