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300517 SE+UE Theory and Application of Confocal Microscopy (2019W)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work



max. 12 participants
Language: German



First-Meeting: Tuesday 1st of October 2019, 18:15 in the seminary room Cell (Ebene 1, Spange 4, UZA I) - attendance is required.
The first part of the course is blocked on 7th, 8th and 9th of October 9:00-18:00; The second part will be a day with working on the Confocal microscope in small groups of 3 or 4 with a selected specimen - date upon accordance. Date for exam and protocol deadline will be upon accordance.


Aims, contents and method of the course

After rapid development, the Confocal Laser Scanning Microscope (CLSM) became the most important imaging tool in both organismic and molecular biology. In this course, we present the theoretical background of CLSM, its application in structure analysis, in measurement and quantification of physiological parameters like membrane potential, ion concentrations or enzyme activities as well as current special techniques. In a hands-on-training, we will apply the following techniques: localisation and identification of structures with organelle specific fluorescent dyes and GFP, 3D-reconstruction and animation, 4D analysis, ratio imaging, spectral dye separation. Participants are encouraged to bring their own samples. Positive examination of this lab course is a prerequisite for independent work on the CLSM of the Cell Imaging and Ultrastructure Research Unit.

Assessment and permitted materials

Active participation in the lab course (30 %), written evaluation of the knowledge acquired in the lectures (30 %), protocol (40 %). Maximum points for the course-evaluation are 100 points. For a positive grade a minimum of 50 points are valid.

Minimum requirements and assessment criteria

The participants know the theoretical background, the possibilities and the limitations of confocal laser scanning microscopy. They are able to work with a modern CLSM and they are familiar with techniques for sample preparation and the evaluation of results.

Examination topics

Lectures on the theoretical background of CLSM, Hands-on-training with provided samples, detailed investigation of a sample selected by the student.

Reading list

Sheppard, C. J. R. & Shotton, D. M. 1997 Confocal laser scanning microscopy. Royal Microscopical Society Microscopy Handbooks. New York: Springer.

Hibbs, A. R. 2004 Confocal microscopy for biologists. New York: Kluwer Academic/Plenum Publishers.

Hepler, P. K. & Gunning, B. E. S. 1998 Confocal fluorescence microscopy of plant cells. Protoplasma 201, 121 - 157.

Lacey, A. J. 1989 Light microscopy in biology: A practical approach. Practical Approach. Oxford: IRL Press.

Association in the course directory


Last modified: Mo 07.10.2019 12:28