Universität Wien FIND

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300517 SE+UE Theory and Application of Confocal Microscopy (2021S)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Moodle

Registration/Deregistration

Details

max. 10 participants
Language: German

Lecturers

Classes

Vorbesprechung: via mail April 2021
Hybridlehre: Vorlesungen online (freie Zeiteinteilung)- Unterichtsmaterial wir dazu verschickt & 2 Präsenztermine (Praxistermine): zu je 5 Stunden vor Ort am Konfokalmikroskop;
Die Präsenztermien werden in 2er Gruppen unter den gegebenen Rahmenbedingungen für Präsenzlehre, aufgrund der Covid-19 Situation, stattfinden. Die Präsenztermine finden frühestens ab Ende April 2021 statt; Alle Präsenz-Termin finden nach Übereinkunft mit den Studierenden statt.

Information

Aims, contents and method of the course

After rapid development, the Confocal Laser Scanning Microscope (CLSM) became the most important imaging tool in both organismic and molecular biology. In this course, we present the theoretical background of CLSM, its application in structure analysis, in measurement and quantification of physiological parameters like membrane potential, ion concentrations or enzyme activities as well as current special techniques. In a hands-on-training, we will apply the following techniques: localisation and identification of structures with organelle specific fluorescent dyes and GFP, 3D-reconstruction and animation, 4D analysis, ratio imaging, spectral dye separation. Participants are encouraged to bring their own samples. Positive examination of this lab course is a prerequisite for independent work on the CLSM of the Cell Imaging and Ultrastructure Research Unit.

Assessment and permitted materials

Active participation in the lab course (30 %), written evaluation of the knowledge aquired in the lectures (30 %), protocol (40 %).
50 % in sum are required for a positive evaluation;

Minimum requirements and assessment criteria

The participants know the theoretical background, the possibilities and the limitations of confocal laser scanning microscopy. They are able to work with a modern CLSM and they are familiar with techniques for sample preparation and the evaluation of results.

Examination topics

Lectures on the theoretical background of CLSM, Hands-on-training with provided samples, detailed investigation of a sample selected by the student.

Reading list

Sheppard, C. J. R. & Shotton, D. M. 1997 Confocal laser scanning microscopy. Royal Microscopical Society Microscopy Handbooks. New York: Springer.

Hibbs, A. R. 2004 Confocal microscopy for biologists. New York: Kluwer Academic/Plenum Publishers.

Hepler, P. K. & Gunning, B. E. S. 1998 Confocal fluorescence microscopy of plant cells. Protoplasma 201, 121 - 157.

Lacey, A. J. 1989 Light microscopy in biology: A practical approach. Practical Approach. Oxford: IRL Press.

Association in the course directory

WZB

Last modified: We 14.04.2021 17:31