Universität Wien

300517 SE+UE Theory and Application of Confocal Microscopy (2023W)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 10 participants
Language: German

Lecturers

Classes

One part of this course (5 lectures and some additional information) will be digital and provided via Moodle. The other (practical) part of this Confocal Microscopy course including intensive hands on training will be on 2 full days in January. Possible course dates are : 4th-5th or 12th-13th of January 2024 (dates will be fixed in accordance with the students), at the University of Vienna, Biology Building (UBB), Djerassiplatz 1, 1030 Vienna, Austria. Additionally - after this block - a written exam and a special application training in small groups will be determined in accordance with the students (6-7 hourse). After the group protocol from the special application training is submitted a final seminary (in the afternoon or evening) will be determined.

Information

Aims, contents and method of the course

After rapid development, the Confocal Laser Scanning Microscope (CLSM) became the most important imaging tool in both organismic and molecular biology. In this course, we present the theoretical background of CLSM, its application in cell biology, molecular biologiy e.g. for structure analysis, for measurement and quantification of physiological parameters like membrane potential, enzyme activities as well as current special techniques. In a hands-on-training, we will apply the following techniques: localisation and identification of structures with organelle specific fluorescent dyes, 3D-reconstruction and animation, 4D analysis, ratio imaging, spectral dye separation. Positive examination of this lab course is a prerequisite for independent work on the CLSM of the Cell Imaging and Ultrastructure Research Unit.

Assessment and permitted materials

Active participation in the lab course (30 %), written evaluation of the knowledge aquired in the lectures (35 %), protocol (35 %) without assitance of AI. A final seminary will be part of "active participation";
50 % in sum are required for a positive evaluation;

Minimum requirements and assessment criteria

The participants know the theoretical background, the possibilities and the limitations of confocal laser scanning microscopy. They are able to work with a modern CLSM and they are familiar with techniques for sample preparation and the evaluation of results.

Examination topics

Lectures on the theoretical background of CLSM, application as a "Hands-on-training" in small groups with provided samples (fixed specimen as well as living cells inculding staining). Independent application in a more intensiv "special application training" in extra small groups at the Confocla Microscope.

Reading list

A diverse literature on confocal microscopy will be provided in the course.
Lectures support material and the presenting slides are written in English language and offered via Moodle. The oral lecture is held in German language. Practical part of the course will be either in German or in English (on demand).

Association in the course directory

MEC-9, MBO 7

Last modified: Mo 18.12.2023 00:03