Universität Wien

300614 VO+SE Theory and Application of Confocal Microscopy (2005W)

Theory and Application of Confocal Microscopy

0.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Vorbesprechung: 4.10.05, 9:00 Uhr, ÜR 7 Anatomie.
Für Studierende ist eine einführende lichtmikroskopische Lehrveranstaltung Vorraussetzung.
Block: 5 Tage im November

Details

Language: German

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Currently no class schedule is known.

Information

Aims, contents and method of the course

Theory and Application of Confocal Microscopy

After rapid development, the Confocal Laser Scanning Microscope (CLSM) became a most important tool in both organismic and molecular biology. In this course, we present:
· the theoretical background of CLSM
· its application in structure analysis
· analysis and quantification of physiological parameters like membrane potential, ion concentration, diffusion of molecules, photosynthesis and respiration

in a hands-on training, we will apply the following techniques:
· localisation and identification of structures with organelle specific fluorescent dyes and antibodies
· 4D analysis
· ratio imaging
· FRET
· FRAP

Participants are encouraged to bring their own samples.

Assessment and permitted materials

Minimum requirements and assessment criteria

Examination topics

Reading list


Association in the course directory

Last modified: Fr 31.08.2018 08:56