300662 SE Seminar to Course III A (2009S)
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Mo 09.02.2009 10:00 to Su 22.02.2009 23:00
- Registration is open from Mo 23.02.2009 01:00 to Su 01.03.2009 23:00
- Deregistration possible until Mo 30.03.2009 23:00
Registration information is available for each group.
Groups
Group 1
Ort: BZB/SR 1, 6.Stock, Dr. Bohrgasse 9, 1030 Wien.Vorbespr: 5.3.09, 17.00 UhrTeilnahme verpflichtend, ThemenvergabeInformationen siehe auch Studentenseite des Zentrums für Molekulare Biologie: http://www.students.mfpl.ac.at/Termine des Seminars (Beginn jeweils 9 Uhr 15, Seminarraum 1, Dr. Bohrgasse 9/6):
18. 5. : Bachmayr-Heyda, Vejdovszky, Zappe
19. 5.: Reinthaler, Halimi, Lippert
22. 5..: Steinkellner, Finkes, Stöger
25. 5.: Hausmann, Anderluh, Gselmann
26. 5.: Paur, Bauer, Fluch
27. 5.: Ramesmayer, Pingger, Pichler
28. 5.: Krenbeck, Kalser, Rohrer
18. 5. : Bachmayr-Heyda, Vejdovszky, Zappe
19. 5.: Reinthaler, Halimi, Lippert
22. 5..: Steinkellner, Finkes, Stöger
25. 5.: Hausmann, Anderluh, Gselmann
26. 5.: Paur, Bauer, Fluch
27. 5.: Ramesmayer, Pingger, Pichler
28. 5.: Krenbeck, Kalser, Rohrer
max. 16 participants
Language: German
Lecturers
Classes
Currently no class schedule is known.
Group 3
Ort: BZB/SR 3, Dr. Bohr-Gasse 9, 1030 Wien.
Teilnahme verpflichtend, Themenvergabe
Informationen siehe auch Studentenseite des Zentrums für Molekulare Biologie: http://www.students.mfpl.ac.at/VORBESPRECHUNG: 5. März, 17.00 Uhr, SR1 6. StockVorläufige Anmeldungen vom 5.März mit Seminarthemenliste:1) Describe the E.coli transformation method(s) and plasmid vectors (such as pUC18/19, pBluescript, pGEM-Teasy or others) by which foreign DNA can be transfered into E.coli.
StudentIn: 05042312) Describe in detail the isolation of the plasmid DNA from E.coli and explain the theoretical basics behind it (use as basis a method provided in the protocol).3) Restriction enzymes and DNA modification enzymes (ligase, DNA polymerase, Klenov, SAP etc.). Theoretical background. Their features and use in DNA analysis and modification (restriction analysis, ligation of DNA fragments etc.) (2 students).
StudentIn: 0600827
06047864) Theoretical background of PCR and applications in recombinant DNA techniques (!!!RT-PCR will be a separate topic!!!!).
StudentIn: 03043125) Describe specification of commonly used E. coli (DH5alpha, XL1-blue, HB101) and yeast strains (such as PJ69-4A, L40, H7Fc) Describe some basic yeast shuttle vectors (pGAD424, pBDcam, pYES), methods of yeast transformation and selection of transformants. Yeast two hybrid assay and Yeast two hybrid screens: theory, applications and plasmid rescue from yeast (group of 3 students).
StudentIn: 05487886) Describe the methods how to introduce foreign DNA into plants, to receive stable transformants. Describe vectors (e.g. pGreen, pBI101) for plant stable transformation and example of application (group of 2 students).
StudentIn: 0604095
05008627) Main features of vectors for plant transient transformations. Methods of transient plant cells transformation.
StudentIn: 05088668) Describe the methods to analyse foreign (transgenic) protein expression in plants (group of 2 students):
------------Western blotting, tagging of proteins: different tags, detection.
StudentIn: 0440213
04401719) Describe methods for gene expression analysis (on the RNA level) in plant(eukaryotic) cells (RT-PCR, cDNA library) (!Northern is a separate topic #12)
StudentIn: 050886410) Microarrays: theory, examples (DNA and oligo chips), CHIP on chip analysis, examples group of 2 students
StudentIn: 0125753
012565511) Southern Blot, Northern Blot and their applications in molecular biology
StudentIn: 0544181
031720212) RNA and DNA in situ hybridisation methods, examples
StudentIn: 040605313) Describe methods of imaging analysis in eukaryotic (plant) cells, examples.
StudentIn: 0502816Literature:
IMP-library (recommended)
Molecular Cloning: A Laboratory Manual (Library Binding)
by Joseph Sambrook (Author), T. Maniatis (Author) "THIS PRINT EDITION OF MOLECULAR CLONING is associated with a Web Site www.MolecularCloning.com
that is evolving into an on-line laboratory manual... Molecular Cloning: A Laboratory Manual (3-Volume Set), Joseph Sambrook, David W. Russell (original by Tom Maniatis)
Very simplistic but helpful: Instant Notes in : Molecular Biology, Genetics, Plant Biology (BIOS Scientific Publishers)
IMP-library (recommended),
WWW Diverse web sites e.g.:
http://www.yeastgenome.org/VL-yeast.html#buds
http://www.fermentas.com/techinfo/appendix/index.html
http://www.neb.com/nebecomm/tech_reference/default.asp
http://www.arabidopsis.org/servlets/Search?action=new_search&type=protocol
ftp://ftp.arabidopsis.org/home/tair/Protocols/Mundy_Protocols.pdf
wikipedia
Teilnahme verpflichtend, Themenvergabe
Informationen siehe auch Studentenseite des Zentrums für Molekulare Biologie: http://www.students.mfpl.ac.at/VORBESPRECHUNG: 5. März, 17.00 Uhr, SR1 6. StockVorläufige Anmeldungen vom 5.März mit Seminarthemenliste:1) Describe the E.coli transformation method(s) and plasmid vectors (such as pUC18/19, pBluescript, pGEM-Teasy or others) by which foreign DNA can be transfered into E.coli.
StudentIn: 05042312) Describe in detail the isolation of the plasmid DNA from E.coli and explain the theoretical basics behind it (use as basis a method provided in the protocol).3) Restriction enzymes and DNA modification enzymes (ligase, DNA polymerase, Klenov, SAP etc.). Theoretical background. Their features and use in DNA analysis and modification (restriction analysis, ligation of DNA fragments etc.) (2 students).
StudentIn: 0600827
06047864) Theoretical background of PCR and applications in recombinant DNA techniques (!!!RT-PCR will be a separate topic!!!!).
StudentIn: 03043125) Describe specification of commonly used E. coli (DH5alpha, XL1-blue, HB101) and yeast strains (such as PJ69-4A, L40, H7Fc) Describe some basic yeast shuttle vectors (pGAD424, pBDcam, pYES), methods of yeast transformation and selection of transformants. Yeast two hybrid assay and Yeast two hybrid screens: theory, applications and plasmid rescue from yeast (group of 3 students).
StudentIn: 05487886) Describe the methods how to introduce foreign DNA into plants, to receive stable transformants. Describe vectors (e.g. pGreen, pBI101) for plant stable transformation and example of application (group of 2 students).
StudentIn: 0604095
05008627) Main features of vectors for plant transient transformations. Methods of transient plant cells transformation.
StudentIn: 05088668) Describe the methods to analyse foreign (transgenic) protein expression in plants (group of 2 students):
------------Western blotting, tagging of proteins: different tags, detection.
StudentIn: 0440213
04401719) Describe methods for gene expression analysis (on the RNA level) in plant(eukaryotic) cells (RT-PCR, cDNA library) (!Northern is a separate topic #12)
StudentIn: 050886410) Microarrays: theory, examples (DNA and oligo chips), CHIP on chip analysis, examples group of 2 students
StudentIn: 0125753
012565511) Southern Blot, Northern Blot and their applications in molecular biology
StudentIn: 0544181
031720212) RNA and DNA in situ hybridisation methods, examples
StudentIn: 040605313) Describe methods of imaging analysis in eukaryotic (plant) cells, examples.
StudentIn: 0502816Literature:
IMP-library (recommended)
Molecular Cloning: A Laboratory Manual (Library Binding)
by Joseph Sambrook (Author), T. Maniatis (Author) "THIS PRINT EDITION OF MOLECULAR CLONING is associated with a Web Site www.MolecularCloning.com
that is evolving into an on-line laboratory manual... Molecular Cloning: A Laboratory Manual (3-Volume Set), Joseph Sambrook, David W. Russell (original by Tom Maniatis)
Very simplistic but helpful: Instant Notes in : Molecular Biology, Genetics, Plant Biology (BIOS Scientific Publishers)
IMP-library (recommended),
WWW Diverse web sites e.g.:
http://www.yeastgenome.org/VL-yeast.html#buds
http://www.fermentas.com/techinfo/appendix/index.html
http://www.neb.com/nebecomm/tech_reference/default.asp
http://www.arabidopsis.org/servlets/Search?action=new_search&type=protocol
ftp://ftp.arabidopsis.org/home/tair/Protocols/Mundy_Protocols.pdf
wikipedia
max. 16 participants
Language: German
Lecturers
Classes
Currently no class schedule is known.
Group 4
Ort: BZB/SR 3, Dr. Bohr-Gasse 9, 1030 Wien.
Teilnahme verpflichtend, Themenvergabe
Informationen siehe auch Studentenseite des Zentrums für Molekulare Biologie: http://www.students.mfpl.ac.at/
Teilnahme verpflichtend, Themenvergabe
Informationen siehe auch Studentenseite des Zentrums für Molekulare Biologie: http://www.students.mfpl.ac.at/
max. 16 participants
Language: German
Lecturers
Classes
Currently no class schedule is known.
Information
Aims, contents and method of the course
Presentation and discussion of special topics of methods used in molecular biology.
Assessment and permitted materials
Minimum requirements and assessment criteria
Examination topics
Reading list
Association in the course directory
BMG 7, BPF 3, B317
Last modified: Mo 07.09.2020 15:44