300721 PP DNA Microarrays (2009S)

Highly parallel molecular analysis of microorganisms

10.00 ECTS (6.00 SWS), SPL 30 - Biologie

Continuous assessment of course work

Erfolgreiche Teilnahme an folgenden LVs ist Voraussetzung:
Einführung in die Mikrobiologie I (300663) + II (300477)
Biodiversität und molekulare Ökologie der Mikroorganismen I (300317) + II (300609)

Vorbesprechung: 03.03.2009 um 10:00 Uhr im HS2
Blockveranstaltung Dauer: 14.09.2009 bis 25.09.2009, 09.00-18.00
Ort: Übungsraum 5, UZA 1, Biozentrum Althanstraße 14, Physiologie 2.211 1.OGÜR 5 Physiologie
Treffpunkt: 14.09.2009, 09.00 im Seminarraum Ökologie

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Registration is open from Mo 09.02.2009 10:00 to Su 22.02.2009 23:00
Deregistration possible until Mo 30.03.2009 23:00

Details

max. 12 participants

Language: German

Lecturers

Alexander Loy

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

This laboratory course will teach attendants how to use DNA microarrays for highly parallel identification of many different microorganisms in environmental and medical samples. The course will provide detailed theoretical and practical knowledge on this high-throughput nucleic acids hybridisation technique; including design, fabrication, and application of diagnostic microarrays, preparation of sample material (fluorescence labelling of nucleic acids), and analysis and interpretation of digital microarray scans.

Assessment and permitted materials

Grading is based on four partial performances: practical lab work, seminar, written examination (1 h), and course protocol. Successful completion of the practical course requires positive grades for all four partial performances.

Minimum requirements and assessment criteria

Learning the practical and theoretical fundamentals of nucleic acid hybridization using the example of the DNA microarray technique (includes e.g. DNA probe design, DNA extraction, PCR, fluorescence labelling of nucleic acids, hybridization, digital image analysis)

Examination topics

Theoretical knowledge will be acquired via lectures, the course script, and original scientific literature; supervision of autonomous practical work in the lab (e.g. DNA extraction, PCR, fluorescence labelling of nucleic acids, hybridization) including computer-based evaluation of lab results; presentation and discussion of scientific papers during the student seminar

Reading list

Association in the course directory

BOE 10, BOE 11, BOE 12, MMI I-2, MMI III-2

Last modified: Mo 07.09.2020 15:44