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301046 UE Laboratory Course I B - Microbiology and Genetics (2020S)

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Yudushkin , Moodle
2 Bücker , Moodle
3 Yudushkin , Moodle
4 Dammermann , Moodle
5 Schlögelhofer , Moodle
6 Schweighofer , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first serve).
Registration information is available for each group.

Groups

Group 1

max. 20 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Reading list

Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1

Group 2

max. 20 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Reading list

T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1

Group 3

max. 20 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Reading list

Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1

Group 4

max. 20 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Reading list

T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1

Group 5

max. 20 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Reading list

T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1

Group 6

max. 20 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Reading list

T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1

Information

Aims, contents and method of the course

Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).

Minimum requirements and assessment criteria

a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).

Examination topics

Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).

Association in the course directory

BMB 1, BMG 1, BBO 2

Last modified: Mo 07.09.2020 15:22