301046 UE Laboratory Course I B - Microbiology and Genetics (2023W)
Continuous assessment of course work
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Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 07.09.2023 14:00 to Th 21.09.2023 18:00
- Deregistration possible until Th 21.09.2023 18:00
Registration information is available for each group.
Groups
Group 1
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, können die entsprechenden Module (Inhalte und Prüfung) über Moodle geöffnet und absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/fuer-studierende/Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students can open and complete the respective modules (content and exam) via Moodle.You can find out more at https://laborlehre-ubb.univie.ac.at/fuer-studierende/All practice rooms may only be entered with a lab coat and closed shoes, and lab glasses must also be purchased - please store private belongings in the cloakroom.
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max. 24 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
see german version
- Monday 02.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 03.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 04.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 05.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 06.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 09.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 10.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 11.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 12.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 13.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Minimum requirements and assessment criteria
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 30%,
- protocol 20%,
- Theoretical basics and final exam 50% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
b. Assessment criteria (key):
- active participation 30%,
- protocol 20%,
- Theoretical basics and final exam 50% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Reading list
Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Group 2
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, können die entsprechenden Module (Inhalte und Prüfung) über Moodle geöffnet und absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/fuer-studierende/Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students can open and complete the respective modules (content and exam) via Moodle.You can find out more at https://laborlehre-ubb.univie.ac.at/fuer-studierende/All practice rooms may only be entered with a lab coat and closed shoes, and lab glasses must also be purchased - please store private belongings in the cloakroom.
_
_
max. 24 participants
Language: German, English
LMS: Moodle
Lecturers
- Gijs Versteeg
- Kathrin Hacker (Student Tutor)
Classes (iCal) - next class is marked with N
see german version
- Monday 02.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 03.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 04.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 05.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 06.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 09.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 10.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 11.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 12.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 13.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Minimum requirements and assessment criteria
UPDATE: 28.09.2023:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 45%; lab book and report (protocol): 10%; final written examination: 45%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 45%; lab book and report (protocol): 10%; final written examination: 45%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
Reading list
Recommended literature:T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3 Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Group 3
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, können die entsprechenden Module (Inhalte und Prüfung) über Moodle geöffnet und absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/fuer-studierende/Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students can open and complete the respective modules (content and exam) via Moodle.You can find out more at https://laborlehre-ubb.univie.ac.at/fuer-studierende/All practice rooms may only be entered with a lab coat and closed shoes, and lab glasses must also be purchased - please store private belongings in the cloakroom.
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_
max. 24 participants
Language: German, English
LMS: Moodle
Lecturers
- Boris Görke
- Andrej Knarr (Student Tutor)
Classes (iCal) - next class is marked with N
see german version
- Monday 30.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 31.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 03.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 06.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 07.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 08.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 09.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 10.11. 08:00 - 11:00 Seminarraum 1.3, Biologie Djerassiplatz 1, 1.005, Ebene 1
- Friday 10.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Minimum requirements and assessment criteria
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Reading list
Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Group 4
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, können die entsprechenden Module (Inhalte und Prüfung) über Moodle geöffnet und absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/fuer-studierende/Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students can open and complete the respective modules (content and exam) via Moodle.You can find out more at https://laborlehre-ubb.univie.ac.at/fuer-studierende/All practice rooms may only be entered with a lab coat and closed shoes, and lab glasses must also be purchased - please store private belongings in the cloakroom.
_
_
max. 24 participants
Language: German, English
LMS: Moodle
Lecturers
- Stephanie Ellis
- Katharina Semrad
- Tamara Civetta (Student Tutor)
Classes (iCal) - next class is marked with N
see german version
- Monday 30.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 31.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 03.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 06.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 07.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 08.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 09.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 10.11. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Minimum requirements and assessment criteria
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Reading list
Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Information
Aims, contents and method of the course
Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.
Assessment and permitted materials
The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).
Examination topics
Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).
Association in the course directory
BMB 1, BMG 1 , BBO 2
Last modified: Tu 07.11.2023 14:28