Universität Wien
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301046 UE Laboratory Course I B - Microbiology and Genetics (2025S)

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.

Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.

-----

max. 20 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

see german version

  • Tuesday 18.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 19.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 20.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 21.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Monday 24.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Tuesday 25.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 26.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 27.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 28.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1

Aims, contents and method of the course

Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).

Minimum requirements and assessment criteria

UPDATE: 09.03.2021:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).

Group 2

Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.

Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.

-----

max. 20 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

see german version

  • Tuesday 18.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 19.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 20.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 21.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Monday 24.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Tuesday 25.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 26.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 27.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 28.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1

Aims, contents and method of the course

Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).

Minimum requirements and assessment criteria

UPDATE: 09.03.2021:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).

Group 3

Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.

Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.

-----

max. 20 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

see german version

  • Tuesday 01.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 02.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 03.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 04.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Monday 07.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Tuesday 08.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 09.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 10.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 11.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1

Aims, contents and method of the course

Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.
Participation in the internship requires strict adherence to laboratory safety regulations. Gross disregard of lab safety rules will result in immediate expulsion from the practical course.

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).
Participation in the practical course requires strict adherence to laboratory safety regulations. Gross disregard to the lab safety rules will result in immediate expulsion from the practical.

Minimum requirements and assessment criteria

A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.

Group 4

Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.

Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.

-----

max. 20 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

see german version

  • Tuesday 11.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
  • Wednesday 12.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
  • Thursday 13.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
  • Friday 14.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
  • Monday 17.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
  • Tuesday 18.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
  • Wednesday 19.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
  • Thursday 20.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
  • Friday 21.03. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien

Aims, contents and method of the course

Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).

Minimum requirements and assessment criteria

UPDATE: 01.04.2024:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 45%; lab book and report (protocol): 10%; final written examination: 45%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Lab book and report: following formal requirements of lab book keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.

**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).

Group 5

Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.

Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.

-----

max. 20 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

see german version

  • Monday 28.04. 13:15 - 14:45 Seminarraum 1.7, Biologie Djerassiplatz 1, 1.010, Ebene 1
  • Tuesday 29.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 30.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 02.05. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Monday 05.05. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Tuesday 06.05. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 07.05. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 08.05. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 09.05. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1

Aims, contents and method of the course

Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).

Minimum requirements and assessment criteria

UPDATE: 09.03.2021:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).

Information

Examination topics

Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).

Reading list

Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1

Association in the course directory

BMB 1, BMG 1, BBO 2

Last modified: Th 23.01.2025 10:26