Universität Wien FIND

Due to the COVID-19 pandemic, changes to courses and exams may be necessary at short notice (e.g. cancellation of on-site teaching and conversion to online exams). Register for courses/exams via u:space, find out about the current status on u:find and on the moodle learning platform.

Further information about on-site teaching and access tests from 21 April 2021 onwards can be found at https://studieren.univie.ac.at/en/info.

301052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2020S)

for diploma, PhD and advanced students

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Gotzmann , Moodle
2 Gotzmann , Moodle

Registration/Deregistration

Registration information is available for each group.

Groups

Group 1

In case non-German speaking students participate we, of course, will teach in English!

max. 8 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Dear Confocal Course Participants,

Yesterday we have been informed that a somehow "normalized" teaching business will not be implemented before MIDST OF MAY!!

All kind of teaching where personal and physical attendance is mandatory - as is true for laboratory courses (this also includes our course) - shall become postponed.

As of today, this means we MUST POSTPONE the course with estimated begin dates in the direction of summer. In clean words, NOT BEFORE JUNE 2020. We were also advised to streamline the course contents and possible have a "shortened" course version in summer.

We DO understand, that this will be true for most of the courses you planned to attend and your time schedule will become tightened.

We cannot come up with a detailed new schedule, as we also have to wait for developments and how our teaching, research obligations will become re-distributed.

Still, I want to come up with two possible schedule solutions in the coming days. Plans for the course for June and July dates will be presented to you. Finally, it will be up to you, if these new dates may be convenient or if you probably want to resign. I want to make sure, that every person resigning due to schedule shift will get a FIXED SEAT in the course in winter semester 2020/2021!

I will come back to you soon with updated schedule suggestions,

Josef Gotzmann

Preliminary Meeting, Tue, March 10th ,2020; 10.30-11.00am (s.t.)
BZB/SemRoom 3, 6th floor MPL main bldg.

ATTENDANCE MANDATORY!!

Registration via u:space for ONE of the two offered courses is SUFFICIENT.

Simple registration does NOT necessarilly lead to a fixed ticket for the course.

In case of over-registration the course head will select people dependent on their career advancement and the actual demand (e.g. for a running thesis)

# of Attendees limited to 8 (per offered group = in total 16).

Details to programme and course workflow in the preliminary meeting.

Course Dates: APRIL 27th,28th,29th; MAY 5th,6th,12th,13th,14th,19th,20th, 2020. Attendance on all days manadatory.

Location: VBC5, Dr.Bohrgasse 9, 1030 Vienna; level E1

Further Info: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

oral exam and continuous assessment

Group 2

In case non-German speaking students participate we, of course, will teach in English!

max. 8 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Dear Confocal Course Participants,

Yesterday we have been informed that a somehow "normalized" teaching business will not be implemented before MIDST OF MAY!!

All kind of teaching where personal and physical attendance is mandatory - as is true for laboratory courses (this also includes our course) - shall become postponed.

As of today, this means we MUST POSTPONE the course with estimated begin dates in the direction of summer. In clean words, NOT BEFORE JUNE 2020. We were also advised to streamline the course contents and possible have a "shortened" course version in summer.

We DO understand, that this will be true for most of the courses you planned to attend and your time schedule will become tightened.

We cannot come up with a detailed new schedule, as we also have to wait for developments and how our teaching, research obligations will become re-distributed.

Still, I want to come up with two possible schedule solutions in the coming days. Plans for the course for June and July dates will be presented to you. Finally, it will be up to you, if these new dates may be convenient or if you probably want to resign. I want to make sure, that every person resigning due to schedule shift will get a FIXED SEAT in the course in winter semester 2020/2021!

I will come back to you soon with updated schedule suggestions,

Josef Gotzmann

Preliminary Meeting, Tue, March10th, 2020; 10.30-11.00am (s.t.)
BZB/SemRoom 3, 6th floor MPL main bldg.

ATTENDANCE MANDATORY!!

Registration via u:space for ONE of the two offered courses is SUFFICIENT.

Simple registration does NOT necessarilly lead to a fixed ticket for the course.

In case of over-registration the course head will select people dependent on their career advancement and the actual demand (e.g. for a running thesis)

# of Attendees limited to 8 (per offered group = in total 16).

Details to programme and course workflow in the preliminary meeting.

Course Dates: APRIL 27th,28th,29th; MAY 5th,6th,12th,13th,14th,19th,20th, 2020. Attendance on all days manadatory.

Location: VBC5, Dr.Bohrgasse 9, 1030 Vienna; level E1

Further Info: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

oral exam and continuous assessmen

Information

Aims, contents and method of the course

Theoretical basis of fluorescence and confocal microscopy
technical description of equipment
Wet lab experience sample preparation
Quality criteria for dealingwith aberrations correction
practical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)

image analysis and deconvolution of selected images

Minimum requirements and assessment criteria

The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

Examination topics

microscopy sample preparation
widefield fluorescence microscopy (incl. Köhler and contrast enhancement)
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008

Association in the course directory

PhD-MB 2, MMEI III, MMB W-2, WZB

Last modified: We 01.04.2020 11:49