301052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2022S)
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 10.02.2022 08:00 to Th 24.02.2022 18:00
- Deregistration possible until Th 24.02.2022 18:00
Registration information is available for each group.
Groups
Group 1
In case non-German speaking students participate we, of course, will teach in English!
max. 8 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Group assignment available via Moodle (March 18)
Presence Teaching planned. Rules and regulations, as well as any changes will be announced in time!Preliminary Meeting (DIGITAL): wed, March 16th, 2022. 10.30-11.00am (sharp!) - digital Zoom meetinghttps://univienna.zoom.us/j/95988147632?pwd=TS9TbER0YktHclllRk1LOFd6M1dmQT09
Attendance mandatory!Registration for ONE of the two offered courses is sufficient!!
Mere registration does not necessarily mean you have a seat in the course fixed!
In case there is more registrants than seats offered the responsible course instructor will select participants based on following criteria: current career status and demand (e.g. for ongoing Master and PhD theses).Limited attendance: 8 people/courseDetails for programme and course schedule will be announced in preliminary meeting and can be viewed at Moodle.Dates: this is all days for both courses!!
Dates (all days) for both courses:
May 16-19; May 23-25; May 31 + June 1
in detail:
Lectures: May 16-18 (both courses)
Hands-on Course 1: May 19 or May 24
Hands-on Course 2: May 24 or 25
Image Processing: May 31 and June 1 (both courses)Location: Max Perutz Labs-Building VBC5-lecel E1- Dr.Bohrgasse 9-A1030 Vienna
Group 2
In case non-German speaking students participate we, of course, will teach in English!
max. 8 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Presence Teaching planned. Rules and regulations, as well as any changes will be announced in time!
Preliminary Meeting (DIGITAL): wed, March 16th, 2022. 10.30-11.00am (sharp!) - digital Zoom meetinghttps://univienna.zoom.us/j/95988147632?pwd=TS9TbER0YktHclllRk1LOFd6M1dmQT09
Attendance mandatory!Registration for ONE of the two offered courses is sufficient!!
Mere registration does not necessarily mean you have a seat in the course fixed!
In case there is more registrants than seats offered the responsible course instructor will select participants based on following criteria: current career status and demand (e.g. for ongoing Master and PhD theses).Limited attendance: 8 people/courseDetails for programme and course schedule will be announced in preliminary meeting and can be viewed at Moodle.Dates: this is all days for both courses!!
Dates (all days) for both courses:
May 16-19; May 24-26; May 31 + June 1
in detail:
Lectures: May 16-18 (both courses)
Hands-on Course 1: May 19 or May 24
Hands-on Course 2: May 25 or 26
Image Processing: May 31 and June 1 (both courses)Location: Max Perutz Labs-Building VBC5-lecel E1- Dr.Bohrgasse 9-A1030 Vienna
Information
Aims, contents and method of the course
Theoretical basis of fluorescence and confocal microscopy technical description of equipment Wet lab experience sample preparationQuality criteria for dealingwith aberrations correctionpractical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)image analysis and deconvolution of selected images
Assessment and permitted materials
continuous assessment
Minimum requirements and assessment criteria
The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.
Examination topics
microscopy sample preparation
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software
Reading list
J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Association in the course directory
MMEI III, MMB W-2, WZB, PhD (SPL 56)
Last modified: Fr 18.03.2022 10:30