Universität Wien

301052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2023S)

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Gotzmann , Moodle
2 Gotzmann , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

In case non-German speaking students participate we, of course, will teach in English!

max. 8 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

THE COURSE WILL BE HELD IN ENGLISH!!

PRELIMINARY MEETING (DIGITAL VIA ZOOM): March 14th, 10.30am
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Confocal Course SS2023 Preliminary Meeting
Time: Mar 14, 2023 10:30 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/66053324801?pwd=UU1LR2Z4WkFKY0hXTFFsdW9Bd2I1dz09
Meeting ID: 660 5332 4801
Passcode: 677929

Course details are announced at Moodle: https://moodle.univie.ac.at/course/view.php?id=368657

Registration via u:space for ONE of the two offered courses (301052-1 or -2) is SUFFICIENT.
Simple registration does NOT necessarily lead to a fixed ticket for the course.
In case of over-registration the course head will select people dependent on their career advancement and the actual demand (e.g. for a running thesis).

# of Attendees limited to 8 (per course; in total 16).

Details to program and course workflow in the preliminary meeting and via Moodle.

To date (Dec 2022) we expect the full course to be held with physical presence. Any changes will become announced in time.

Course Dates: MAY, 9th-11th, 16th+17th, 23rd + 24th, 30th + 31st.
Physical attendance at all course dates mandatory!

Location: Max Perutz Labs; VBC5, level E1, Dr.Bohrgasse 9, 1030 Vienna;

IMPORTANT INFORMATION (March 9th, 2023).
This year the course is heavily over-booked with 40 applicants for the 16 seats. Based on this situation, I am forced to apply the official SPL rules:
- Preference for students of the master studies UA 865, 830, 834, 877 – IRRESPECTIVE of the points bet.
- Students of the master studies UA 865, 830, 834, 877 will be ranked according to points bet
- Then all other studies, ranked by the points bet

For the summer term 2023 I am happy to announce, I can offer 4 more groups for 8 additional students.
More details in the preliminary meeting on March 14th.

Group 2

In case non-German speaking students participate we, of course, will teach in English!

max. 8 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

THE COURSE WILL BE HELD IN ENGLISH!!

PRELIMINARY MEETING (DIGITAL VIA ZOOM): March 14th, 10.30am
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Confocal Course SS2023 Preliminary Meeting
Time: Mar 14, 2023 10:30 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/66053324801?pwd=UU1LR2Z4WkFKY0hXTFFsdW9Bd2I1dz09
Meeting ID: 660 5332 4801
Passcode: 677929

Course details are announced at Moodle: https://moodle.univie.ac.at/course/view.php?id=368657

Registration via u:space for ONE of the two offered courses (301052-1 or -2) is SUFFICIENT.
Simple registration does NOT necessarily lead to a fixed ticket for the course.
In case of over-registration the course head will select people dependent on their career advancement and the actual demand (e.g. for a running thesis).

# of Attendees limited to 8 (per course; in total 16).

Details to program and course workflow in the preliminary meeting and via Moodle.

To date (Dec 2022) we expect the full course to be held with physical presence. Any changes will become announced in time.

Course Dates: MAY, 9th-11th, 16th+17th, 23rd + 24th, 30th + 31st.
Physical attendance at all course dates mandatory!

Location: Max Perutz Labs; VBC5, level E1, Dr.Bohrgasse 9, 1030 Vienna;

IMPORTANT INFORMATION (March 9th, 2023).
This year the course is heavily over-booked with 40 applicants for the 16 seats. Based on this situation, I am forced to apply the official SPL rules:
- Preference for students of the master studies UA 865, 830, 834, 877 – IRRESPECTIVE of the points bet.
- Then all other studies, ranked by the points bet

For the summer term 2023 I am happy to announce, I can offer 4 more groups for 8 additional students.
More details in the preliminary meeting on March 14th.

Information

Aims, contents and method of the course

Theoretical basis of fluorescence and confocal microscopy technical description of equipment Wet lab experience sample preparationQuality criteria for dealingwith aberrations correctionpractical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)image analysis and deconvolution of selected images

Assessment and permitted materials

continuous assessment

Minimum requirements and assessment criteria

The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

Examination topics

microscopy sample preparation
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006Yuste, Imaging; A Laboratory manual; CSHL-Press, 2010D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008

Association in the course directory

MMEI III, MMB W-2, WZB, PhD (SPL 56) MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:

Last modified: Tu 18.04.2023 09:50