Universität Wien

301052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2024S)

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Gotzmann , Moodle
2 Gotzmann , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

In case non-German speaking students participate we, of course, will teach in English!

max. 12 participants
Language: English
LMS: Moodle

Lecturers

Classes

TEACHING LANGUAGE OF THE COURSE IS ENGLISH!!!!

PRELIMINARY MEETING (DIGITAL VIA ZOOM): March, 12th, 2024; 10h30am
ATTENDANCE AT THE PRELIMINARY MEETING IS MANDATORY!!!!

Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting Confocal Course SS2024
Time: Mar 12, 2024 10:30 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/62667009400?pwd=SzhaVmVTNWN3eVp5M0RRSjZyUVFYUT09
Meeting ID: 626 6700 9400
Passcode: 077117

Course details will be announced in Moodle: https://moodle.univie.ac.at/course/view.php?id=423424

!! REGISTRATION VIA U:SPACE FOR ONE OF THE OFFERED COURSES (301052-1 OR -2) IS SUFFICIENT!!
!! REGISTRATION VIA U:SPACE DOES NOT AUTOMATICALLY LEAD TO COURSE PARTICIPATION!

IN CASE OF OVER-BOOKING THE ACTUAL SPL-RULES WILL BECOME APPLIED:
- The first 20 seats will be assigned to students of the Master-Program (865, 830, 834 and 877).
- Seat allocation will be preformed according to the Vamount of the points bid.
- A quota-rule for the remianing 4 seats (2 seats/course) will become enacted for students forom studies with a natural science background
- allocation of seats according to the amount of points bid
- @ points: if a vacant seat cannot become allocated based on points (e.g. same # of points bid), then the date of registration will decide (the earlier the better)

Seat limitation: 12 (per course; in total for both courses 24 seats)

Details for course programme, schedule and workflow in the preliminary meeting.
Seat allocation will be done shortly after the preliminary meeting.
Further details and information will then be distributed via Moodle.
Any changes will be announced in time!

COURSE DATES: VALID FOR BOTH COURSES: May 6th-8th; May 14th+15th; May 22nd + 23rd; May 28th + 29th, 2024
An attendance at all these dates is MANDATORY!

Group 2

In case non-German speaking students participate we, of course, will teach in English!

max. 12 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

TEACHING LANGUAGE OF THE COURSE IS ENGLISH!!!!

PRELIMINARY MEETING (DIGITAL VIA ZOOM): March, 12th, 2024; 10h30am
ATTENDANCE AT THE PRELIMINARY MEETING IS MANDATORY!!!!

Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting Confocal Course SS2024
Time: Mar 12, 2024 10:30 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/62667009400?pwd=SzhaVmVTNWN3eVp5M0RRSjZyUVFYUT09
Meeting ID: 626 6700 9400
Passcode: 077117

Course details will be announced in Moodle: https://moodle.univie.ac.at/course/view.php?id=423424

!! REGISTRATION VIA U:SPACE FOR ONE OF THE OFFERED COURSES (301052-1 OR -2) IS SUFFICIENT!!
!! REGISTRATION VIA U:SPACE DOES NOT AUTOMATICALLY LEAD TO COURSE PARTICIPATION!

IN CASE OF OVER-BOOKING THE ACTUAL SPL-RULES WILL BECOME APPLIED:
- The first 20 seats will be assigned to students of the Master-Program (865, 830, 834 and 877).
- Seat allocation will be preformed according to the Vamount of the points bid.
- A quota-rule for the remianing 4 seats (2 seats/course) will become enacted for students forom studies with a natural science background
- allocation of seats according to the amount of points bid
- @ points: if a vacant seat cannot become allocated based on points (e.g. same # of points bid), then the date of registration will decide (the earlier the better)

Seat limitation: 12 (per course; in total for both courses 24 seats)

Details for course programme, schedule and workflow in the preliminary meeting.
Seat allocation will be done shortly after the preliminary meeting.
Further details and information will then be distributed via Moodle.
Any changes will be announced in time!

COURSE DATES: VALID FOR BOTH COURSES: May 6th-8th; May 14th+15th; May 22nd + 23rd; May 28th + 29th, 2024
An attendance at all these dates is MANDATORY!

Information

Aims, contents and method of the course

Theoretical basis of fluorescence and confocal microscopy technical description of equipment Wet lab experience sample preparationQuality criteria for dealingwith aberrations correctionpractical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)image analysis and deconvolution of selected images

Assessment and permitted materials

continuous assessment

Minimum requirements and assessment criteria

The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

Examination topics

microscopy sample preparation
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006Yuste, Imaging; A Laboratory manual; CSHL-Press, 2010D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008

Association in the course directory

MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:, PhD (SPL 56)

Last modified: Mo 15.04.2024 09:46