Universität Wien
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301052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2025S)

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 N.N. , Moodle
2 N.N. , Moodle

Registration/Deregistration

Groups

Group 1

In case non-German speaking students participate we, of course, will teach in English!

max. 12 participants
Language: English
LMS: Moodle

Lecturers

    Classes

    TEACHING LANGUAGE OF THE COURSE IS ENGLISH!!!!

    PRELIMINARY MEETING (DIGITAL VIA ZOOM-see below): March 18th, 2025, 10h30am
    ATTENDANCE AT THE PRELIMINARY MEETING IS MANDATORY!!!!
    Josef Gotzmann is inviting you to a scheduled Zoom meeting.
    Topic: Preliminary Meeting Confocal Course SS2025
    Time: Mar 18, 2025 10:30 AM Vienna
    Join Zoom Meeting
    https://univienna.zoom.us/j/67175260198?pwd=sfYtss4UKgg1MOlmNT0sz0lYfzkkEX.1
    Meeting ID: 671 7526 0198
    Passcode: 670191

    Course details will be announced in Moodle: https://moodle.univie.ac.at/course/view.php?id=441147

    !! REGISTRATION VIA U:SPACE FOR ONE OF THE OFFERED COURSES (301052-1 OR -2) IS SUFFICIENT!!
    !! REGISTRATION VIA U:SPACE DOES NOT AUTOMATICALLY LEAD TO COURSE PARTICIPATION!

    IN CASE OF OVER-BOOKING THE ACTUAL SPL-RULES WILL BECOME APPLIED:
    - The first 20 seats will be assigned to students of the Master-Program (865, 830, 834 and 877).
    - Seat allocation will be performed according to the amount of the points bid.
    - A quota-rule for the remaining 4 seats (2 seats/course) will become enacted for students from studies with a natural science background
    - allocation of seats according to the amount of points bid
    - @ points: if a vacant seat cannot become allocated based on points (e.g. same # of points bid), then the date of registration will decide (the earlier the better)

    Seat limitation: 12 (per course; in total for both courses 24 seats)

    Details for course program, schedule and workflow in the preliminary meeting.
    Seat allocation will be done shortly (2-3 days) after the preliminary meeting.
    Further details and information will then be distributed via Moodle.
    Any changes will be announced in time!

    COURSE DATES: VALID FOR BOTH COURSES: MAY 5,6,8,13,14,20,21,26,and 28th, 2025
    An attendance at ALL these dates is MANDATORY!

    Group 2

    In case non-German speaking students participate we, of course, will teach in English!

    max. 12 participants
    Language: German, English
    LMS: Moodle

    Lecturers

      Classes

      TEACHING LANGUAGE OF THE COURSE IS ENGLISH!!!!

      PRELIMINARY MEETING (DIGITAL VIA ZOOM-see below): March 18th, 2025, 10h30am
      ATTENDANCE AT THE PRELIMINARY MEETING IS MANDATORY!!!!
      Josef Gotzmann is inviting you to a scheduled Zoom meeting.
      Topic: Preliminary Meeting Confocal Course SS2025
      Time: Mar 18, 2025 10:30 AM Vienna
      Join Zoom Meeting
      https://univienna.zoom.us/j/67175260198?pwd=sfYtss4UKgg1MOlmNT0sz0lYfzkkEX.1
      Meeting ID: 671 7526 0198
      Passcode: 670191

      Course details will be announced in Moodle: https://moodle.univie.ac.at/course/view.php?id=441147

      !! REGISTRATION VIA U:SPACE FOR ONE OF THE OFFERED COURSES (301052-1 OR -2) IS SUFFICIENT!!
      !! REGISTRATION VIA U:SPACE DOES NOT AUTOMATICALLY LEAD TO COURSE PARTICIPATION!

      IN CASE OF OVER-BOOKING THE ACTUAL SPL-RULES WILL BECOME APPLIED:
      - The first 20 seats will be assigned to students of the Master-Program (865, 830, 834 and 877).
      - Seat allocation will be performed according to the amount of the points bid.
      - A quota-rule for the remaining 4 seats (2 seats/course) will become enacted for students from studies with a natural science background
      - allocation of seats according to the amount of points bid
      - @ points: if a vacant seat cannot become allocated based on points (e.g. same # of points bid), then the date of registration will decide (the earlier the better)

      Seat limitation: 12 (per course; in total for both courses 24 seats)

      Details for course program, schedule and workflow in the preliminary meeting.
      Seat allocation will be done shortly (2-3 days) after the preliminary meeting.
      Further details and information will then be distributed via Moodle.
      Any changes will be announced in time!

      COURSE DATES: VALID FOR BOTH COURSES: MAY 5,6,7,13,14,20,21,26,and 28th, 2025
      An attendance at ALL these dates is MANDATORY!

      Information

      Aims, contents and method of the course

      Theoretical basis of fluorescence and confocal microscopy technical description of equipment Wet lab experience sample preparationQuality criteria for dealingwith aberrations correctionpractical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)image analysis and deconvolution of selected images.

      At the beginning of the course there will be a quiz to screen the existing knowledge on the course topic. The same quiz will be conducted after your course attendance, so that all students can evaluate the learning effect from the course.

      Assessment and permitted materials

      continuous assessment

      Minimum requirements and assessment criteria

      The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

      Examination topics

      microscopy sample preparation
      measuring point spread function and chromatic aberration
      confocal microscopy
      image processing using "huygens" and ImageJ software

      Reading list

      J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006Yuste, Imaging; A Laboratory manual; CSHL-Press, 2010D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008

      Association in the course directory

      MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:, PhD (SPL 56)

      Last modified: Fr 10.01.2025 12:26