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301135 UE Laboratory Course II - Biochemistry (2021W)

5.00 ECTS (5.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Summary

1 Slade , Moodle
2 Teige , Moodle
3 Hamilton , Moodle
4 Bachmair , Moodle
5 Teige , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 16 participants
Language: English
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Group 2

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Group 3

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Group 4

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays (kinetics, inhibitor studies, allosteric activation), SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Group 5

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays (kinetics, inhibitor studies, allosteric activation), SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Information

Assessment and permitted materials

Compulsory attendance;
the evaluation consists of different performances:
- active participation and results obtained,
- written report,
- final exam

Minimum requirements and assessment criteria

a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).

Examination topics

Content and Background of the experiments carried out during the lab course

Reading list

Course script and comprehensive theoretical supplements to the topics of the course on Moodle

Association in the course directory

BMB 3, BMG 3

Last modified: Tu 28.09.2021 12:49