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301135 UE Laboratory Course II - Biochemistry (2025W)
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Groups
Group 1
max. 18 participants
Language: English
Lecturers
- Markus Teige
- Florian Halhammer (Student Tutor)
Classes
see german version
Aims, contents and method of the course
Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.
Group 2
max. 18 participants
Language: German
Lecturers
- Markus Teige
- Jan Emanuel Wimmer (Student Tutor)
Classes
see german version
Aims, contents and method of the course
Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.
Group 3
max. 18 participants
Language: German
Lecturers
- Sascha Martens
- Karma Abdoun (Student Tutor)
Classes
see german version
Aims, contents and method of the course
Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.
Group 4
max. 18 participants
Language: German
Lecturers
- Flora Haider
- Elena Jedipour (Student Tutor)
Classes
see german version
Aims, contents and method of the course
Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays (kinetics, inhibitor studies, allosteric activation), SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.
Information
Assessment and permitted materials
Compulsory attendance;
the evaluation consists of different performances:
- active participation and results obtained,
- written report,
- final exam
the evaluation consists of different performances:
- active participation and results obtained,
- written report,
- final exam
Minimum requirements and assessment criteria
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Examination topics
Content and Background of the experiments carried out during the lab course
Reading list
Course script and comprehensive theoretical supplements to the topics of the course on Moodle
Association in the course directory
BMB 3, BMG 3
Last modified: Fr 27.06.2025 00:02