Universität Wien FIND

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301151 UE Practical Course in fluorescence-/confocal microscopy including image processing (2017W)

for diploma, PhD and advanced students

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Gotzmann, Moodle
2 Gotzmann, Moodle

Registration/Deregistration

Groups

Group 1

max. 8 participants
Language: German
LMS: Moodle

Lecturers

Classes

Preliminary Meeting, Fr, Oct.6th, 10.30-11.00am (s.t.)
BZB/SemRoom 3, 6th floor MFPL main bldg.
ATTENDANCE MANDATORY!!
Registration via UniVis/u:space for one of the two offered courses is SUFFICIENT.
Simple registration does not necessarilly lead to a fixed ticket for the course.

In case of over-registration the course head will select people dependent on their career advancement and the actual demand for e.g. a running thesis

Course Dates: November 13th, 14th, 15th, 21st, 22nd, 27th, 29th, 30th; December 5th+6th. Attendance on all days manadatory.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Group 2

max. 8 participants
Language: German
LMS: Moodle

Lecturers

Classes

Preliminary Meeting, Fr, Oct.6th, 10.30-11.00am (s.t.)
BZB/SemRoom 3, 6th floor MFPL main bldg.
ATTENDANCE MANDATORY!!
Registration via UniVis/u:space for one of the two offered courses is SUFFICIENT.
Simple registration does not necessarilly lead to a fixed ticket for the course.

In case of over-registration the course head will select people dependent on their career advancement and the actual demand for e.g. a running thesis

Course Dates: November 13th, 14th, 15th, 21st, 22nd, 27th, 29th, 30th; December 5th+6th. Attendance on all days manadatory.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Information

Aims, contents and method of the course

Theoretical basis of fluorescence and confocal microscopy

technical description of equipment

practical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)

2 days of Image Processing: Deconvolution of acquired images; Introduction into Image J

Minimum requirements and assessment criteria

The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

Examination topics

microscopy sample preparation
widefield fluorescence microscopy (incl. Köhler and contrast enhancement)
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008

Association in the course directory

PhD-MB 2, WZB, MMB W-2, MMEI III

Last modified: Fr 31.08.2018 08:43