Universität Wien
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301151 UE Practical Course in fluorescence-/confocal microscopy including image processing (2022W)

for diploma, PhD and advanced students

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Summary

1 Gotzmann , Moodle
2 Gotzmann , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 8 participants
Language: German
LMS: Moodle

Lecturers

Classes

THE COURSE WILL BE HELD IN ENGLISH!!

PRELIMINARY MEETING (DIGITAL VIA ZOOM): Wed, Oct 12th, 10.00am
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting Confocal Course WS22-23
Time: Oct 12, 2022 10:00 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/63096703978?pwd=a1FmRFlrdEhGZmZpZEVEWUVGcTMrUT09
Meeting ID: 630 9670 3978
Passcode: 322921

Course details are announced at Moodle: https://moodle.univie.ac.at/course/view.php?id=328477

Registration via u:space for ONE of the two offered courses (301151-1 or -2) is SUFFICIENT.
Simple registration does NOT necessarily lead to a fixed ticket for the course.
In case of over-registration the course head will select people dependent on their career advancement and the actual demand (e.g. for a running thesis).

# of Attendees limited to 8 (per course; in total 16).

Details to program and course workflow in the preliminary meeting.

To date (June 2022) we expect the full course to be held with physical presence. Any changes will become announced in time.

Course Dates: Nov 15th-17th; Nov 22nd + 23rd; Nov 29th and 30th; -25th; Dec 5th + 6th.
Physical attendance at all course dates mandatory!

Location: Max Perutz Labs; VBC5, level E1, Dr.Bohrgasse 9, 1030 Vienna;

Group 2

max. 8 participants
Language: German
LMS: Moodle

Lecturers

Classes

THE COURSE WILL BE HELD IN ENGLISH!!

PRELIMINARY MEETING (DIGITAL VIA ZOOM): Wed, Oct 12th, 10.00am
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting Confocal Course WS22-23
Time: Oct 12, 2022 10:00 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/63096703978?pwd=a1FmRFlrdEhGZmZpZEVEWUVGcTMrUT09
Meeting ID: 630 9670 3978
Passcode: 322921

Course details are announced at Moodle: https://moodle.univie.ac.at/course/view.php?id=328477

Registration via u:space for ONE of the two offered courses (301151-1 or -2) is SUFFICIENT.
Simple registration does NOT necessarily lead to a fixed ticket for the course.
In case of over-registration the course head will select people dependent on their career advancement and the actual demand (e.g. for a running thesis).

# of Attendees limited to 8 (per course; in total 16).

Details to program and course workflow in the preliminary meeting.

To date (June 2022) we expect the full course to be held with physical presence. Any changes will become announced in time.

Course Dates: Nov 15th-17th; Nov 22nd + 23rd; Nov 29th and 30th; -25th; Dec 5th + 6th.
Physical attendance at all course dates mandatory!

Location: Max Perutz Labs; VBC5, level E1, Dr.Bohrgasse 9, 1030 Vienna;

Information

Aims, contents and method of the course

Theoretical basis of fluorescence and confocal microscopy

technical description and training for usage of equipment

sample preparation (practical)
quality criteria and correction of aberrations

SELF-CONTAINED practical microscopy of selected samples by confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)

2 days of Image Processing: Analysis and deconvolution of acquired images; Introduction into Image J

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, continuous assessment of theoretical knowledge (50%/20%/30%); each of the sub-grades must have a positive evaluation.

Minimum requirements and assessment criteria

The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

Examination topics

microscopy sample preparation
theory of widefield fluorescence microscopy
measuring point spread function and chromatic aberration
theoretical and practical aspects of confocal microscopy
image processing

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Molecular Biology of the Cell, Alberts, Garland Sciences,
Review series on „Imaging in Cell Biology“ in Nature Cell Biology Vol 5 (2003) Supplement
Review series on „Biological Imaging“ in Science Vol 300 (2003), 82-99
Eric C. Wait, Michael A. Reiche, Teng-Leong Chew: “Hypothesis-driven quantitative fluorescence microscopy – the importance of reverse-thinking in experimental design”, Journal of Cell Science 2020 133: jcs250027 doi: 10.1242/jcs.250027
Jonkman, J., Brown, C.M., Wright, G.D. et al. Guidance for quantitative confocal microscopy. Nat Protoc (2020). https://doi.org/10.1038/s41596-020-0307-7
https://www.ibiology.org/online-biology-courses/microscopy-series/

Association in the course directory

PhD-MB 2, WZB, MMB W-2, MMEI III, MMB IV.

Last modified: Tu 06.12.2022 14:29