Universität Wien

301171 UE Course III A - Laboratory Work in Molecular Biology (2021S)

10.00 ECTS (8.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Summary

1 Witte , Moodle
2 Schweighofer , Moodle
3 Sonnleitner , Moodle
4 Campbell , Moodle
5 Schweighofer , Moodle
6 Witte , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 16 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Preliminary meeting: March 10, 2021, 1 p.m., online via Zoom (link will be sent by e-mail)

Date: 17.5. - 4.6.2021
Compulsory seminar 301172!

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 2

max. 16 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

mandatory organizational meeting: Friday 12th March 2021, 2 p.m till 4 p.m
online via Zoom (links will be sent per email to registered students and students on the waiting list)

date lab course: 17.5 - 28.5.2021

accompanying seminar (301172) is mandatory

place: BZB,/ÜR, Dr. Bohrgasse 9, 1030 Wien

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 3

max. 16 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

-vor Ort Lehre-
verpflichtende Vorbesprechung online 22.3.2020 15:00 Uhr
Termin: 7.6. - 18.6.2021
ÜR/BZB, Dr.Bohrgasse 9,1030 Wien.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (20%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (20%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):

Group 4

max. 16 participants
Language: English
LMS: Moodle

Lecturers

Classes

Mandatory organizational meeting: 10.3.21 at 10:00 via Zoom.
https://univienna.zoom.us/j/98807833169?pwd=bGZGUTBRSkdpajdkZUhESkZzdm00UT09
Meeting ID: 988 0783 3169
Passcode: 229201

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 5

max. 16 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

mandatory organizational meeting: Friday 12th March 2021, 2 p.m till 4 p.m.
online via Zoom (links will be sent per email to registered students and students on the waiting list)

date lab course: 7.- 18.6.2021

accompanying mandatory seminar (301172)
place: BZB,/ÜR, Dr. Bohrgasse 9, 1030 Wien.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 6

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

Preliminary meeting: March 10, 2021, 1 p.m., online via Zoom (link will be sent by e-mail)

Date: 26.07.-06.08.2021
Compulsory seminar 301172!


Information

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.

Examination topics

Contents and theoretical background of the course units.

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Association in the course directory

BMG 5, WZB

Last modified: Mo 12.07.2021 10:09