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301171 UE Course III A - Laboratory Work in Molecular Biology (2023S)
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 09.02.2023 08:00 to Th 23.02.2023 18:00
- Deregistration possible until Th 23.02.2023 18:00
Registration information is available for each group.
Groups
Group 1
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
see german version
Aims, contents and method of the course
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Group 2
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
mandatory preliminary meeting:
Friday 10th March 2023, 16:00 hSeminar room SR1/2 (room 6.501/6.504)Dr. Bohrgasse 9, 1030 Wiendates lab practical: 17.04. - 05.05.2023lab practical only together with seminar SE 301172place: 6th floor teaching laboratories, Dr. Bohrgasse 9, 1030 Vienna
check also department website: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Group 3
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
see german version
mandatory preliminary meeting: March 20th 10:00 SR1/2
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (20%)
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):Permitted material for written exam: calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):Permitted material for written exam: calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (20%)
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):
Group 4
max. 18 participants
Language: English
LMS: Moodle
Lecturers
Classes
Premeeting: 12.4.23, 11:00, Seminar Room 3 (6.507), Dr.Bohrgasse 9, Max Perutz Labs
The course will take place from July 10 - July 28Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Group 5
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
mandatory preliminary meeting:
Friday 10th March 2023, 16:00 hSeminar room SR1/2 (room 6.501/6.504)Dr. Bohrgasse 9, 1030 Wiendates lab practical: 29.05. - 16.06.2023lab practical only together with seminar SE 301172 !place: 6th floor teaching laboratories, Dr. Bohrgasse 9, 1030 Vienna
check also department website: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Group 6
max. 18 participants
Language: German
LMS: Moodle
Lecturers
Classes
Termin: 29.5. - 16.6.2023 ganztägig
Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien
Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
RNA isolation from E. coli, RT, qPCR, CrispR, ribozymes
Assessment and permitted materials
presence during practical course compulsory, participating in the lab work, protocol of the results, written exam at the end
Information
Examination topics
Contents and theoretical background of the course units.
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Association in the course directory
BMG 5, WZB
Last modified: Fr 26.05.2023 00:06
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.