Universität Wien

301171 UE Course III A - Laboratory Work in Molecular Biology (2024S)

10.00 ECTS (8.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Schweighofer , Moodle
2 Witte , Moodle
3 Sonnleitner , Moodle
4 Semrad , Moodle
5 Schweighofer , Moodle
6 Campbell , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory preliminary meeting: Thursday 14th March 2024, 15:30h Seminar room 1, Dr.Bohrgasse 9/6501-6504

Lab course dates: 8.04. - 26.04.2024 whole day

Place: ÜR/BZB, Dr.Bohrgasse 9,1030 Wien
Accompanying Seminar 301172 mandatory !
Ort: ÜS 1,Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Please see also department website for students: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 2

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Verpflichtende Vorbesprechung: Termin folgt
Termin: 13.- 31.5.2024 ganztägig
Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

Begleitendes Seminar 301172 verpflichtend !

Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 3

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Verpflichtende Vorbesprechung am 6.3.2024, 10:00, SR1, Dr. Bohrgasse 9
Termin: 8.- 26.4.2024 ganztägig

Begleitendes Seminar 301172 verpflichtend !

Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (20%)
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):

Permitted material for written exam: calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (20%)
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):

Group 4

max. 18 participants
Language: English
LMS: Moodle

Lecturers

Classes

Verpflichtende Vorbesprechung: Termin folgt
Termin: 29.4.- 17.5..2024 ganztägig
Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

Begleitendes Seminar 301172 verpflichtend !

Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 5

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory preliminary meeting: Thursday 14th March 2024, 15:30h Seminar room 1, Dr.-Bohr-Gasse 9/6501-6504

Lab course dates: 21.05. - 07.06.2024 whole day

Place: ÜR/BZB, Dr.Bohrgasse 9,1030 Wien

Accompanying Seminar 301172 mandatory !

Ort: ÜS 1,Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Please see also department website for students: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 6

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory premeeting on April 10th at 14:00 online:
https://univienna.zoom.us/j/65517137098?pwd=MWdiemtHOVp5bTFxczBzWjFkYXcvUT09

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Information

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.

Examination topics

Contents and theoretical background of the course units.

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Association in the course directory

BMG 5

Last modified: Th 13.06.2024 14:26