Universität Wien
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301171 UE Course III A - Laboratory Work in Molecular Biology (2024W)

for the specialization Microbiology/Genetics

10.00 ECTS (8.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Schweighofer , Moodle
Tu 01.10. 13:30-14:30 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
2 Witte , Moodle
3 Sonnleitner , Moodle
We 02.10. 10:00-11:00 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
4 Semrad , Moodle
5 Schweighofer , Moodle
Tu 01.10. 13:30-14:30 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 18 participants
Language: German, English
LMS: Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
    Register/Deregister for this course

    Lecturers

    Classes (iCal) - next class is marked with N

    Mandatory preliminary meeting: Tuesday 1st October 2024 13:30h Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504

    Lab course dates: 14.10. - 1.11.2024 whole day

    Place: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 Vienna
    Accompanying Seminar 301172 mandatory !

    Please see also department website for students: http://molekularebiologie.univie.ac.at/

    Aims, contents and method of the course

    Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.

    Assessment and permitted materials

    Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

    Minimum requirements and assessment criteria

    a. Minimum requirement: participation during the internship, writing a protocol, exam, b. Assessment standard: participation 50%, minutes 30%, exam 20%

    Examination topics

    Content of the course held

    Reading list

    Course documents in Moodle Information on the literature available on the slides in Moodle.

    Group 2

    max. 18 participants
    Language: German, English
    LMS: Moodle

    Registration/Deregistration

    Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
      Register/Deregister for this course

      Lecturers

      Classes

      Termin: 4.- 22.11.2024 ganztägig
      Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

      Begleitendes Seminar 301172 verpflichtend !

      Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
      Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

      Aims, contents and method of the course

      Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
      Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.

      Assessment and permitted materials

      Compulsory attendance; the evaluation consists of different performances:
      +) active participation and results obtained (10%)
      +) verbal examination and discussion (daily) (30%)
      +) written protocol (10%)
      +) written exam (20%):
      +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
      Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

      each of the subgrades must have a positive evaluation.

      Minimum requirements and assessment criteria

      +) active participation and results obtained (10%)
      +) verbal examination and discussion (daily) (30%)
      +) written protocol (10%)
      +) written exam (20%):
      +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

      Examination topics

      Content of the course held

      Reading list

      Course documents in Moodle Information on the literature available on the slides in Moodle.

      Group 3

      max. 18 participants
      Language: German, English
      LMS: Moodle

      Registration/Deregistration

      Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
        Register/Deregister for this course

        Lecturers

        Classes (iCal) - next class is marked with N

        Verpflichtende Vorbesprechung am 2.10.2024 10:00 SR1
        Termin: 4.- 22.11.2024 ganztägig
        Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

        Begleitendes Seminar 301172 verpflichtend !

        Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
        Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

        • Monday 04.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Tuesday 05.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Wednesday 06.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Thursday 07.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Friday 08.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Monday 11.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Tuesday 12.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Wednesday 13.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Thursday 14.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Friday 15.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Monday 18.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Tuesday 19.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Wednesday 20.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Thursday 21.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
        • Friday 22.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien

        Aims, contents and method of the course

        Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar:
        e.g. Cloning and expression of certain genes in bacteria including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern blotting; expression analysis of reporter genes

        Maximal number of participants: 18

        Assessment and permitted materials

        Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

        Minimum requirements and assessment criteria

        Minimum requirements for passing the course: Attendance, protocol, final exam.

        Assessment criteria: participation 50%, protocol 30%, written exam 20%

        Examination topics

        Contents and theoretical background of the course units.

        Reading list

        Current Literature and background information will be made available on Moodle

        Group 4

        max. 18 participants
        Language: German, English
        LMS: Moodle

        Registration/Deregistration

        Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
          Register/Deregister for this course

          Lecturers

          Classes

          Termin: 30.9.-18.10.24 ganztägig
          Ort: ÜR/BZB, Dr.Bohrgasse 9, 1030 Wien.
          Begleitendes Seminar 301172 verpflichtend !
          Ort: ÜS 1,Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
          Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/
          verpflichtende Vorbesprechung:-WICHTIG-Terminänderung: 27. 9.; 9:00 - 10:30; Dr. Bohrgasse 9/6 (6th. floor), 1030 Wien; SR 2 (Room 6.507)

          Aims, contents and method of the course

          Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteins Maximal number of participants: 16

          Assessment and permitted materials

          Compulsory attendance; the evaluation consists of different performances:

          +) active participation and results obtained (10%)
          +) verbal examination and discussion (daily) (30%)
          +) written protocol (10%)
          +) written exam (20%):
          +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

          Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

          each of the subgrades must have a positive evaluation.

          Minimum requirements and assessment criteria

          +) active participation and results obtained (10%)
          +) verbal examination and discussion (daily) (30%)
          +) written protocol (10%)
          +) written exam (20%):
          +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

          Examination topics

          Contents and theoretical background of the course units

          Reading list

          Current Literature and background information will be made available on Moodle

          Group 5

          max. 18 participants
          Language: German, English
          LMS: Moodle

          Registration/Deregistration

          Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
            Register/Deregister for this course

            Lecturers

            Classes (iCal) - next class is marked with N

            Mandatory preliminary meeting: Tuesday 1st October 2024 13:30h Seminar Room 1 Dr.Bohrgasse 9/6.501-6.504

            Lab course dates: 25.11. - 13.12.2024 whole day

            Place: Teaching Lab D Dr.-Bohr-Gasse 9/6.526 A-1030 Vienna
            Accompanying Seminar 301172 mandatory !

            Please see also department website for students: http://molekularebiologie.univie.ac.at/

            Aims, contents and method of the course

            Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
            Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.

            Assessment and permitted materials

            Compulsory attendance; the evaluation consists of different performances:
            +) active participation and results obtained (10%)
            +) verbal examination and discussion (daily) (30%)
            +) written protocol (10%)
            +) written exam (20%):
            +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
            Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

            each of the subgrades must have a positive evaluation.

            Minimum requirements and assessment criteria

            +) active participation and results obtained (10%)
            +) verbal examination and discussion (daily) (30%)
            +) written protocol (10%)
            +) written exam (20%):
            +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

            Examination topics

            Content of the course held

            Reading list

            Course documents in Moodle Information on the literature available on the slides in Moodle.

            Association in the course directory

            BMG 5

            Last modified: We 11.09.2024 11:06