Universität Wien
Warning! The directory is not yet complete and will be amended until the beginning of the term.

301171 UE Course III A - Laboratory Work in Molecular Biology (2025S)

10.00 ECTS (8.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

Registration/Deregistration

Groups

Group 1

max. 18 participants
Language: German, English

Lecturers

    Classes

    Mandatory preliminary meeting:
    Tuesday 4th March 2025 15:00h Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504

    Lab course dates: 24.03. - 11.04.2025 whole day

    Place: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 Vienna
    Accompanying Seminar 301172 mandatory !

    Please see also department website for students: http://molekularebiologie.univie.ac.at/

    Assessment and permitted materials

    Compulsory attendance; the evaluation consists of different performances:

    +) active participation and results obtained (10%)
    +) verbal examination and discussion (daily) (30%)
    +) written protocol (10%)
    +) written exam (20%):
    +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

    Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

    each of the subgrades must have a positive evaluation.

    Minimum requirements and assessment criteria

    +) active participation and results obtained (10%)
    +) verbal examination and discussion (daily) (30%)
    +) written protocol (10%)
    +) written exam (20%):
    +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

    Group 2

    max. 18 participants
    Language: German, English

    Lecturers

      Classes

      Verpflichtende Vorbesprechung: Termin folgt
      Termin: 19.5. - 6.6.2025 ganztägig
      Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

      Begleitendes Seminar 301172 verpflichtend !

      Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
      Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

      Assessment and permitted materials

      Compulsory attendance; the evaluation consists of different performances:

      +) active participation and results obtained (10%)
      +) verbal examination and discussion (daily) (30%)
      +) written protocol (10%)
      +) written exam (20%):
      +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

      Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

      each of the subgrades must have a positive evaluation.

      Minimum requirements and assessment criteria

      +) active participation and results obtained (10%)
      +) verbal examination and discussion (daily) (30%)
      +) written protocol (10%)
      +) written exam (20%):
      +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

      Group 3

      max. 18 participants
      Language: German, English

      Lecturers

        Classes (iCal) - next class is marked with N

        Verpflichtende Vorbesprechung: 3.3.2025 10:00 SR1
        Termin: 24.3.-11.4.2025 ganztägig
        Begleitendes Seminar 301172 verpflichtend !

        Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
        Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

        • Monday 24.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Tuesday 25.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Wednesday 26.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Thursday 27.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Friday 28.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Monday 31.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Tuesday 01.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Wednesday 02.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Thursday 03.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Friday 04.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Monday 07.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Tuesday 08.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Wednesday 09.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Thursday 10.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
        • Friday 11.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien

        Assessment and permitted materials

        Compulsory attendance; the evaluation consists of different performances:

        +) active participation and results obtained (20%)
        +) verbal/written examination and discussion (daily) (30%)
        +) written protocol (30%)
        +) written exam (20%):

        Permitted material for written exam: calculator (internet access and smartphones are not allowed)

        each of the subgrades must have a positive evaluation.

        Minimum requirements and assessment criteria

        +) active participation and results obtained (20%)
        +) verbal/written examination and discussion (daily) (30%)
        +) written protocol (30%)
        +) written exam (20%):

        Group 4

        max. 18 participants
        Language: English

        Lecturers

          Classes

          Verpflichtende Vorbesprechung: Termin folgt
          Termin: 28.4. - 16.5.2025 ganztägig
          Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

          Begleitendes Seminar 301172 verpflichtend !

          Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
          Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

          Assessment and permitted materials

          Compulsory attendance; the evaluation consists of different performances:

          +) active participation and results obtained (10%)
          +) verbal examination and discussion (daily) (30%)
          +) written protocol (10%)
          +) written exam (20%):
          +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

          Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

          each of the subgrades must have a positive evaluation.

          Minimum requirements and assessment criteria

          +) active participation and results obtained (10%)
          +) verbal examination and discussion (daily) (30%)
          +) written protocol (10%)
          +) written exam (20%):
          +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

          Group 5

          max. 18 participants
          Language: German, English

          Lecturers

            Classes

            Mandatory preliminary meeting:
            Tuesday 4th March 2025 15:00h Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504

            Lab course dates: 09.06. - 27.06.2025 whole day

            Place: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 Vienna
            Accompanying Seminar 301172 mandatory !

            Please see also department website for students: http://molekularebiologie.univie.ac.at/

            Assessment and permitted materials

            Compulsory attendance; the evaluation consists of different performances:

            +) active participation and results obtained (10%)
            +) verbal examination and discussion (daily) (30%)
            +) written protocol (10%)
            +) written exam (20%):
            +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

            Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

            each of the subgrades must have a positive evaluation.

            Minimum requirements and assessment criteria

            +) active participation and results obtained (10%)
            +) verbal examination and discussion (daily) (30%)
            +) written protocol (10%)
            +) written exam (20%):
            +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

            Group 6

            max. 18 participants
            Language: German, English

            Lecturers

            Classes

            Mandatory premeeting on 12.03.2025, 15:00, online:
            https://univienna.zoom.us/j/69146852020?pwd=Xlsaz0sHYZdD6X2ReRLaF9XDDvmXxg.1
            Course duration: 30.6. - 18.7.2025 all day
            Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

            The accompanying Seminar 301172 is required !

            Assessment and permitted materials

            Compulsory attendance; the evaluation consists of different performances:

            +) active participation and results obtained (10%)
            +) verbal examination and discussion (daily) (30%)
            +) written protocol (10%)
            +) written exam (20%):
            +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

            Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

            each of the subgrades must have a positive evaluation.

            Minimum requirements and assessment criteria

            +) active participation and results obtained (10%)
            +) verbal examination and discussion (daily) (30%)
            +) written protocol (10%)
            +) written exam (20%):
            +) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

            Information

            Aims, contents and method of the course

            Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
            Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.

            Examination topics

            Contents and theoretical background of the course units.

            Reading list

            Course documents in Moodle Information on the literature available on the slides in Moodle.

            Association in the course directory

            BMG 5

            Last modified: Fr 10.01.2025 00:02