Universität Wien

301180 UE Advanced genetics: C.elegans (2023S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: English

Lecturers

Classes

Dates: 20 - 31.3.2023 all day.
Prerequisites and Admission Criteria:
1. Attendance at the preliminary meeting (13 March, 2pm, online)
2. Master's students in the Master's programs A066877 Genetik und Entwicklungbiologie, A066834 Molekulare Biologie and A066865 Molecular Biology (focus areas Cellular Architecture and Maintenance and Chromosome and RNA Biology) will be given priority in the allocation of spaces.
3. For students in A066865 Molecular Biology, successful completion of lecture series 301401 Concepts in Molecular Biology is required.
4. For students who did not complete their bachelor's degree at the University of Vienna in Mikrobiologie und Genetik (BMG) or Molekulare Biologie (BMB): Students who have received other practical courses (UE) with mandatory attendance as requirements at the time of admission to the program must pass those in advance (take a copy of the admission letter and a current transcript with you to the preliminary meeting).


Information

Aims, contents and method of the course

2 week advanced course for the genetic model system C. elegans, covering classical forward and reverse genetics, as well as more recent methods of targeted genome editing. Topics:
- Introduction to C. elegans as an experimental model
- Classical forward genetics incl. genetic mapping and epistasis
- Reverse genetics (RNAi)
- Targeted genome manipulation by homologous recombination
- Cellular analysis by immunofluorescence microscopy
- Seminars on current C. elegans research at the Vienna Biocenter campus

A basic understanding of genetics is essential for this course. Completion of the following introductory lecture series is therefore highly recommended: 301557 VO Principles in Genetics and Developmental Biology (WS).

Assessment and permitted materials

Grading is based on class participation including carrying out course experiments and written protocol (~20 pages). There will be no final exam. Instead students are each responsible for one of the course experiments.

Minimum requirements and assessment criteria

Attendance mandatory (taking time off during the course only possible with the prior agreement of the instructors and if compatible with ongoing experiments). Grading is as follows: Class participation: 16 (40%) points; written protocol: 16 points (40%); supervision of course experiment: 8 points (20%). Each part must be completed successfully for a passing grade.
Grading scheme:
36-40 points: very good (1)
31-35 points: good (2)
26-30 points: satisfactory (3)
20-25 points: sufficient (4)
<19 points: insufficient (5)

Examination topics

There will be no final exam. Instead students will be responsible in pairs for one of the course experiments. Key here is the successful orientation of the class for the day’s experiments, the collection of results and their interpretation. Written protocols should include a brief introduction to each experiment, a description of methods and results as well as a discussion of results obtained (if any). Evaluation of course participation includes active participation in class discussion and carrying out experiments.

Reading list

Course manual. This will be distributed several weeks before the course. Individual topics will be covered in detail on the blackboard during the course.

Association in the course directory

MGE I-2, MGE III-2, MMB III-3a:

Last modified: Mo 23.10.2023 09:08