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301234 VO Advanced Biochemical and Biophysical Methods (2018S)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie


Language: English

Examination dates



Vorbesprechung: 2.3.2018, 14.00 Uhr
VBC5 lecture hall A (Rm. 1.216)
05.03; 09:00-10:30 Leonard Introductory lecture (including structure-function relationship and protein-protein interactions)
05.03, 10:45-12:15 Leonard From protein properties to purification
06.03; 09:00-10:30 Slade Protein expression and purification
06.03; 10:45-12:15 Stolt-Bergner Integral membrane proteins and protein complexes
07.03; 09:00-10:30 Sedivy Protein quality assessment and control
07.03; 10:45-12:15 Zagrovic Math primer
Sedivy Dong Introduction to X-ray crystallography, SLS and SAXS (part 1)
09.03; 10:45-12:15 Dong Introduction to X-ray crystallography, SLS and SAXS (part 2)
14.03; 09:00-10:30 Sedivy Absorption spectroscopy
14.03; 10:45-12:15 Yudushkin Fluorescence spectroscopy
16.03; 09:00-10:30 Sedivy Applications of absorption and fluorescence spectroscopy
16.03; 10:45-12:15 Hartl Introduction to mass spectroscopy
19.03; 09:00-10:30 Sedivy Microcalorimetry
19.03; 10:45-12:15 Yudushkin Introduction to light microscopy
20.03; 09:00-10:30 Blaas Introduction to electron microscopy
20.03; 10:45-12:15 Yudushkin Imaging methods for detection of molecular proximity
21.03; 09:00-10:30 VBCF tour 1 (upon demand)
21.03; 10:45-12:15 VBCF tour 2 (upon demand)


Aims, contents and method of the course

This theoretical course will start with introductory lectures on the basic concepts in structural biology and cover modern approaches and fundamentals of the methods used in recombinant protein expression, purification, biophysical characterization and quality assessment of macromolecular samples as well as cell biophysics approaches. The covered topics include static and dynamic light scattering, fundamentals of X-ray crystallography, absorption and fluorescence spectroscopy techniques (CD, FRET, FLIM, FC(C)S, fluorescence anisotropy, etc), mass spectroscopy and imaging methods (light and electron microscopy).

Assessment and permitted materials

Verpflichtende Anwesenheit. Die Beurteilung basiert auf folgenden Teilleistungen: Qualität und Aufbereitung der Seminarpräsentation, aktive Teilnahme an den Diskussionen der vorgestellten Publikationen, schriftliche Beiträge (die prozentuelle Aufteilung der Teilleistungen wird vom Kursleiter bekanntgegeben). Jede der Teilleistungen muss positiv sein.

Minimum requirements and assessment criteria

Provide a comprehensive overview of strategies and methods for protein expression and purification; enable students to choose a better strategy for a given protein
Highlight the utility of different methods of protein characterization for understanding its structure and function using specific examples
Explain theoretical background and instrumentation used for protein characterization in amount sufficient for critical interpretation of original research results
Enable students to choose an adequate method for a specific question

Examination topics

Reading list

Association in the course directory


Last modified: Mo 07.09.2020 15:44