Universität Wien

301253 UE Molecular Biology Laboratory Course III (Cell Biology) (2020W)

5.00 ECTS (5.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Summary

1 Propst , Moodle
2 Fuchs , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 16 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Practical course to learn cell biological techniques such as propagating mammalian cells under sterile conditions, cell culture techniques, transfection, fixation and staining of cells, flow cytometry, phase contrast, immunofluorescence and time lapse microscopy and to analyze the basic behavior of cells during the cell cycle, mitosis, differentiation, apoptosis, and the analysis of the cytoskeleton.

Assessment and permitted materials

The evaluation is based on 3 sub grades:
1) Active participation in conducting the experiments and knowledge and understanding of the details of the experimental proceedings; this features with 50% in the final grade.
2) A written report of the experiments conducted and the results obtained, to be submitted within 1 month of the end of the course; this features with 25% in the final grade.
3) A written exam on the last day of the course; this features with 25% in the final grade.
Minimal requirement for the positive evaluation of the exam: 62.5% of the attainable points.
Language of the exam: German or English if needed.
Tools permitted at the exam: pocket calculator.

Minimum requirements and assessment criteria

Mandatory attendance; positive evaluation in each of the 3 sub grades;
Minimal requirement for the positive evaluation of the exam: 62.5% of the attainable points.

Examination topics

Theoretical background knowledge as described in the supplied script and as discussed in the daily introductions and evaluations of the results as well as the knowledge about the details of the experiments.

Reading list

A script with detailed description of the experimental proceedings and additional information will be supplied.

Group 2

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Aims: Students acquire the basic techniques of cell biology. Practical course to learn cell biological techniques such as propagating mammalian cells under sterile conditions, transfection, fixation, staining, phase contrast and immunofluorescence microscopy and to analyze the basic behavior of cells during the cell cycle, differentiation, apoptosis, oncogenic transformation, and the analysis of the cytoskeleton.

Assessment and permitted materials

Compulsory attendance; (1) Practical participation in the experiments; 50% share of the grading (2) Written report of the experiments and their results; 25% share of the grading (3) Final examination; 25% share of the grading. Allowed aids: pocket calculator

Minimum requirements and assessment criteria

Minimum requirements for positive assessment of the written test: 50%.
Assessment criteria:
100-87,5%: very good (1)
87,4-75%: good (2)
74,9-62,5%: satisfactory (3)
62,4-50%: sufficient (4)
< 50%: insufficient (5)

Examination topics

Examination topics: The theoretical basics stated in the work instructions of the course and the contents discussed in the daily preliminary meetings and discussions of the results.

Reading list

Script (will be provided on the first day of the course)
Textbook "Molecular Biology of the Cell" Wiley-Verlag (German edition) or "Molecular Biology of the Cell" Garland Science (English edition) in the respective latest edition.

Association in the course directory

BMB 4, BIO II-3

Last modified: We 21.04.2021 11:27