Universität Wien
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301271 VO Principles and practice of advanced microscopic technques (2019W)

life sciences applications using theory and case studies

2.00 ECTS (1.00 SWS), SPL 30 - Biologie
Moodle

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Details

Language: English

Examination dates

Lecturers

Classes (iCal) - next class is marked with N

Dates: October 22nd,23rd,24th, 28th,30th, 2019 each 1.30-4.00pm (s.t.);
Location: MFPL SemRoom1/2, 6th floor;

Exam Dates for winter term 2019/20:
all exams MFPL, sem room3, 3rd floor
all exams 2.00-3.30pm (s.t.)
Dec 12th, 2019
Feb 6th, 2020
Mar 5th, 2020
Apr 22nd, 2020

  • Tuesday 22.10. 13:30 - 16:00 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
  • Wednesday 23.10. 13:30 - 16:00 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
  • Thursday 24.10. 13:30 - 16:00 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
  • Monday 28.10. 13:30 - 16:00 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
  • Wednesday 30.10. 13:30 - 16:00 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien

Information

Aims, contents and method of the course

It is the aim of this lecture to support people with microscopy experience (basic knowledge in biooptics recommended and expected) to get involved in advanced microscopy techniques and how these can be applied for the life sciences. The major focus will be set to transfer theoretical background knowledge into applied experimental setups. This translational aspect will be trained using case-studies.
Keywords: microscopy, optics, contrasting techniques, imaging, image processing, live imaging, fluorescence, superresolution microscopy, sample preparation, cameras and detection, optical highlighters

Assessment and permitted materials

Written exam

Minimum requirements and assessment criteria

each exam: 6 questions for 6 points each (max 36 points); 18 points needed for positive grade

Examination topics

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
https://www.ibiology.org/online-biology-courses/microscopy-series/

Association in the course directory

MMB III-2, MMB W-2, MGE III-1, PhD MB 3, MMEI III, UF MA BU 01, UF MA BU 04

Last modified: Mo 07.09.2020 15:22