301271 VO Principles and practice of advanced microscopic technques (2021W)

life sciences applications using theory and case studies

MIXED

Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

Language: English

Examination dates

Lecturers

Josef Gotzmann

Classes

UPDATE (Sept 30): From 1 October 2021 onwards, the 2,5G rule applies: Participation in on-site courses and exams is subject to being PCR-tested, vaccinated or having recovered from COVID-19 (antigen tests are no longer valid) as stipulated by the City of Vienna.
******************************************************************UPDATE (Sept29): Due to COVID rules, it is mandatory to REGISTER for a physical attendance! I have created a "poll" in Moodle, where all students shall participate and select at which dates they plan to attend physically. Note: the lecture room can be filled with 30 people in accordance with the COVID-19 rules!
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As of today (Sept 29) the lectures will be held in a HYBRID format!!
Any changes will be announced via Moodle.
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Dates:
27.10./Oct 27th: 13.30-16.00/1.30-4.00pm (s.t.)
28.10./Oct 28th: 14.00-16.30/2.00-4.30pm (s.t.)
29.10./Oct 29th: 11.00-13.30/11.00am-1.30pm (s.t.)
2.11./Nov 2nd: 14.30-17.00/2.30-5.00pm (s.t.)
3.11./Nov 3rd: 13.30-16.00/1.30-4.00pm (s.t.)
PRESENCE TEACHING:
Location: MPL SemRoom1/2, 6th floor
DIGITAL TEACHING: digital lectures AT THE SAME DATES AS ANNOUNCED. Session links will be provided via Moodle.

Exam Dates for winter term 2021/22:
All exams will be conducted in a "Presence Mode", if DIGITALLY, then will be held in an "OPEN BOOK" format!
All exams 2.00-3.30pm (s.t.)
December 15th, 2021
February, 3rd, 2022
March 2nd, 2022
April 5th, 2022

Information

Aims, contents and method of the course

It is the aim of this lecture to support people with microscopy experience (basic knowledge in biooptics recommended and expected) to get involved in advanced microscopy techniques and how these can be applied for the life sciences. The major focus will be set to transfer theoretical background knowledge into applied experimental setups. This translational aspect will be trained using case-studies.
Keywords: microscopy, optics, contrasting techniques, imaging, image processing, live imaging, fluorescence, superresolution microscopy, sample preparation, cameras and detection, optical highlighters

Assessment and permitted materials

Written exam

Minimum requirements and assessment criteria

each exam: 6 questions for 6 points each (max 36 points); 18 points needed for positive grade

Examination topics

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
https://www.ibiology.org/online-biology-courses/microscopy-series/

Association in the course directory

MMB III-2, MMB W-2, MGE III-1, PhD MB 3, MMEI III, UF MA BU 01, UF MA BU 04

Last modified: Tu 01.03.2022 09:10