Universität Wien

301272 VO+UE Live imaging course (2018S)

for diploma, PhD and advanced students

1.00 ECTS (1.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 6 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

Preliminary Meeting: March 8th,2018, 10.00-10.30 am (sharp); BZB/SR 3-6.Stock, Dr.Bohrgasse 9.1030 Wien. Attendance mandatory!

Course Dates: June 19+20th, 2018 (VBC5 level E1); 9.00 a.m. - 6.00 p.m.

LIMITED ATTENDANCE: 6 people
Simple U:Space registration is NOT necessarily a ticket for the course. In case of overbooking the head of the course will select people on the basis of career status and the demand on performing microscopic experiments. Students with experience in light microscopy AND those who have a proven demand for their master/PhD-work will be preferentially selected.
LIMITED ATTENDANCE: 6 people
Details on the Course will be given in the preliminary meeting.
Course Location: MFPL-VBC5, level E1, Dr. Bohrgasse 9, 1030 Vienna

  • Thursday 08.03. 10:00 - 10:30 BZB EDV-Raum CCR01, 6.Ebene 6.505, Dr.-Bohr-Gasse 9, 1030 Wien

Information

Aims, contents and method of the course

The Live Imaging workshop intends to give students a theoretical and practical input on important considerations of live imaging using modern light microscopic technologies.

- Lecture 1: Live Imaging Considerations
- Lecture 2: Live Imaging Dyes and Fluorophores
hands-on planning of overnight and individual shorter time lapse experiments using widefield, spinning disc and confocal microscope setups

Assessment and permitted materials

Continuous Assessment/Oral exam

Minimum requirements and assessment criteria

Students shall learn the theoretical background of imaging in a live regimen. This includes knowledge on instrumental and technological theory, combined with considerations on practical do's and don'ts. The latter shall finally be applied in practical hands-on-sessions using two different live microscopy setups.

Examination topics

a. Students will understand the theory behind basic and advanced light microscopy technologies
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will have to learn and work in cooperative teams

Reading list

Yuste, Live Imaging; A Laboratory manual; CSHL-Press, 2010
Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011
Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011
Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009
http://www.microscopyu.com/
http://www.olympusmicro.com/index.html
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.leica-microsystems.com/science-lab/

Association in the course directory

PhD-MB 2, MMEI III, MMB W-2, WZB

Last modified: We 30.11.2022 00:27