Universität Wien

301272 VO+UE Live imaging course (2023S)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: English

Lecturers

Classes

see german version

Information

Aims, contents and method of the course

The Live Imaging workshop intends to give students a theoretical and practical input on important considerations of live imaging using modern light microscopic technologies, like confocal, spinning disc, fluorescence widefield, superresolution and high-content imaging microscopy. A session of data handling complements the microscope-related data acquisition.
Five basic lectures:
- Lecture 1: Live Imaging Considerations and Techniques overview
- Lecture 2: Sample Preparation
- Lecture 3: Live Imaging Dyes and Fluorophores-basics and applications
- Lecture 4: Special techniques: FRAP, AIRY Scanning, Lattice SIM
- Lecture 5: Handling Live Imaging Data

Three days of extensive hands-on sessions, planning and executing overnight and individual shorter time lapse experiments using various available microscope setups. The course includes half a day of computer hands-on to train handling of live imaging data (includes "file formats and data structure" / "necessary corrections" / "prepare video material for publications")

Assessment and permitted materials

Continuous Assessment

Minimum requirements and assessment criteria

Students shall learn the theoretical background of imaging in a live regimen. This includes knowledge on instrumental and technological theory, combined with considerations on practical do's and don'ts. The latter shall finally be applied in extended practical hands-on-sessions using different live microscopy setups.

Examination topics

a. Students will understand the theory behind basic and advanced light microscopy technologies
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will be taught how to handle live imaging data in terms of processing and analysis
f. Students will learn and work in cooperative teams

Reading list

Yuste, Live Imaging; A Laboratory manual; CSHL-Press, 2010
Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011
Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011
Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009
www.fpbase.org
https://www.ibiology.org/talks/live-cell-imaging/
http://www.microscopyu.com/
https://www.olympus-lifescience.com/de/microscope-resource/
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.leica-microsystems.com/science-lab/
https://www.rms.org.uk/resources-downloads/online-microscopy-talks.html

Association in the course directory

MMEI III, MMB W-2, WZB, PhD (SPL 56), MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:

Last modified: Fr 02.06.2023 09:48