301273 VO+UE Live imaging course (2022W)
Continuous assessment of course work
Labels
ON-SITE
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 08.09.2022 14:00 to Th 22.09.2022 18:00
- Deregistration possible until Th 22.09.2022 18:00
Details
max. 12 participants
Language: English
Lecturers
Classes
see "German" version
Information
Aims, contents and method of the course
Assessment and permitted materials
Continuous Assessment
Minimum requirements and assessment criteria
Students shall learn the theoretical background of imaging in a live regimen. This includes knowledge on instrumental and technological theory, combined with considerations on practical do's and don'ts. The latter shall finally be applied in extended practical hands-on-sessions using different live microscopy setups. Eminently important is to prepare students for handling the data with respect to size, processing and analyses.
Examination topics
a. Students will understand the theory behind basic and advanced light microscopy technologies
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will learn and work in cooperative teams
f. Students will get a comprehensive picture by being introduced into data management
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will learn and work in cooperative teams
f. Students will get a comprehensive picture by being introduced into data management
Reading list
Yuste, Live Imaging; A Laboratory manual; CSHL-Press, 2010Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009www.fpbase.orghttps://www.ibiology.org/talks/live-cell-imaging/http://www.microscopyu.com/https://www.olympus-lifescience.com/de/microscope-resource/http://zeiss-campus.magnet.fsu.edu/index.htmlhttp://www.leica-microsystems.com/science-lab/https://www.rms.org.uk/resources-downloads/online-microscopy-talks.html
Association in the course directory
MMEI III, MMB W-2, WZB, PhD (SPL 56)
Last modified: Mo 22.08.2022 11:30
The Live Imaging workshop intends to give students a theoretical and practical input on important considerations of live imaging using advanced light microscopic technologies, like confocal, spinning disc, fluorescence widefield, high-content and even superresolution imaging microscopy.
Two basic lectures:
- Lecture 1: Live Imaging Considerations
- Lecture 2: Live Imaging Dyes and FluorophoresExtensive hands-on planning of overnight and individual shorter time lapse experiments using various available microscope setups.
An afternoon is dedicated to give expert advice on how to handle live imaging data - this will include selected examples as well as insight into advanced processing pipelines, including aspects of Artificial Intelligence.