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301432 UE RNA Profiling in Microbiology/Immunology (2019W)

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work



max. 15 participants
Language: English



Vorbesprechung: Dienstag, 01.10.2019, 15-16 Uhr
Ort: Veterinärmedizinische Universität Wien, Veterinärplatz 1, Gebäude GA, 1. Stock, Glasbox neben Hörsaal D
Lehrveranstaltungsblock von Donnerstag 03.10. bis Montag 14.10.2019 jeweils 9.30 bis 17.30 Uhr
Einführende Vorlesung und Skript zu Lehrveranstaltungsbeginn


Aims, contents and method of the course

The course communicates theoretical and practical knowledge necessary to quantify nucleic acid sequences using reverse-transcription quantitative PCR (RT-qPCR), but also digital PCR, the current gold standard for precise counting of (small) DNA molecule numbers. Examples from the fields of microbiology and immunology will be addressed. Major experimental topics are the use of reference sequences like spike-in controls for molecular diagnostics, "normalisers" required to determine relative transcript expression and counting of absolute numbers of DNA copies. Practical training includes primer design for monitoring of targets like mRNA variants, miRNAs and DNAs by a fluorescent dye or various probe formats. The course also aims to develop basic skills and abilities in statistical evaluation and graphical presentation of the data, as well as trains storytelling in written communication. In 2019 the course has its 20th anniversary. The course is designed for master students of the final trimester. max. 15 students

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: answers in an initial questionnaire (questions are based on information provided by the review article: Kralik P, & Ricchi M, 2017 A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything. Front Microbiol 8: 108), active participation and results obtained, theoretical knowledge including final colloquium, oral presentation of a methods article and written protocol report (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Examination topics

Reading list

Association in the course directory


Last modified: Tu 15.10.2019 08:48