Universität Wien

301484 UE Practical Course in Molecular Microbiology, Microbial Ecology and Immunobiology (2023W)

Diversity and function of uncultured microbes in medical and environmental samples

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, können die entsprechenden Module (Inhalte und Prüfung) über Moodle geöffnet und absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/fuer-studierende/

Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.

There will be a mandatory safty instruction for this course. After the registration period, the admitted students can open and complete the respective modules (content and exam) via Moodle.You can find out more at https://laborlehre-ubb.univie.ac.at/fuer-studierende/

All practice rooms may only be entered with a lab coat and closed shoes, and lab glasses must also be purchased - please store private belongings in the cloakroom.
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Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 14 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

A preparatory meeting for the course will take place on the 29th of January at 1 pm via Zoom.

The course will run from February 19th to March 1st (Monday-Friday) from 9:00 am to 5:00 pm.

  • Monday 19.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Tuesday 20.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Wednesday 21.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Thursday 22.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Friday 23.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Monday 26.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Tuesday 27.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Wednesday 28.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Thursday 29.02. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Friday 01.03. 08:00 - 18:15 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1

Information

Aims, contents and method of the course

Course goals: In this course you will learn modern methods fur studying microorganisms without cultivation, including how to identify microorganisms and visualize them directly in environmental samples and how to study the activity and function of organisms in complex microbial communities.

Places in the course will be assigned based on the set points, the requirements of the different master programs and waiting time.

Assessment and permitted materials

Evaluation will have three parts: 1) practical work in the lab, 2) seminars, and 3) written report. Participants will need to pass all three evaluations to pass the course.

Minimum requirements and assessment criteria

Active participation in lab experiments and seminar (incl. presentation), as well as written protocol.

Examination topics

Methods: Theoretical knowledge will be disseminated through lectures (PowerPoint Presentations), the course handout and original literature. Supervised hands-on training in independent experiments will teach you how to identify microorganisms through 16S rRNA gene/transcript analysis and fluorescence in situ hybridization techniques (FISH/ CARD-FISH). You will further learn about the use of functional genes/transcripts to identify microorganisms involved in a certain function, and how to investigate their activity with stable isotope probing incubations. The theoretical principles of high-resolution secondary ion mass spectrometry (NanoSIMS) and Raman microspectroscopy coupled to stable isotope incubations will also be discussed.

The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.

Reading list

Literature will be provided during the course.

Association in the course directory

MMEI I

Last modified: Mo 13.11.2023 10:09