Universität Wien
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301555 PP Advanced course in microbiology and genetics (2025W)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Details

max. 10 participants
Language: German, English

Lecturers

Classes

Students should contact listed supervisors for the exact timing, as this depends which others students are currently present in the labs.
Topics and Methods:
- Bücker: cloning of enhancer sequences into a targeting vector, generation of cell lines and analysis of transcription changes based on enhancer integration; Molecualr cloning, mammalian tissue culture
- Dammermann: In this project you will help characterize the molecular mechanisms underlying centrosome function. This will involve generating plasmid DNA constructs by standard cloning, C. elegans microinjection and genetic crosses and widefield epifluorescence microscopy. Standard molecular and cell biological techniques, C. elegans culture and manipulation
- Horn: The project will focus on viruses or bacteria infecting microbial eukaryotes and includes the characterization of infection cycles and the analysis of host-microbe interactions. microbiology and molecular biology techniques, protist/cell culture, (fluorescence) microscopy
- Valenta (2 positions): Molecular biology, protein expression & purification, biochemistry, basophil activation
Wöbken: In this project, we will isolate and identify bacteria from desert soils. microbiology and molecular biology techniques, bacterial cultivation, 16S rRNA gene sequencing for identification

Information

Aims, contents and method of the course

During this practical course the participants will deal with selected examples of experiments related to the field of molecular microbiology. The methods used are mainly, but not exclusively those acquired during their studies. Design and execution of experiments, documentation and discussion of results have to be summarized in a protocol. Topic: Post-transcriptional regulation of gene expression in bacteria, protein-protein-, RNA-protein-, RNA-RNA-interactions.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Compulsory attendance;
- active participation (discussion contributions, participation, ….): 20%
- results of the performed experiments: 10%
- theoretical understanding and practical knowledge of the methods used: 15%
- documentation and interpretation of the results: 15%
- written final report (bachelor thesis): 40%

Examination topics

Content and scientific background of the performed experiments.

Reading list

Topic-specific literature will be provided.

Association in the course directory

BMG 11

Last modified: Fr 27.06.2025 00:02