Universität Wien
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301557 PP Abschlussarbeit im Schwerpunkt Molekulare Biologie (2025W)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Details

max. 10 participants
Language: German, English

Lecturers

Classes

Students should contact listed supervisors for the exact timing, as this depends which others students are currently present in the labs.
Topics and methods:
- Matos: The project is about DNA repair mechanisms. standard molecular and cell biological techniques
- Abouelezz (Ries lab): In this project, you will label mammalian cell lines using fluorescent probes, acquire images, and analyze the results. Mammalian cell culture, fluorescence microscopy, image analysis
- Dong: The project will focus mainly on expression, purification and characterization of recombinant proteins. Molecular biology, protein expression & purification, biochemistry
- Raible: In this project, you will help to identify and characterize genetic mutants in laboratory populations of bristle worms injected with genomic editing constructs; this involves tissue dissection, genomic PCR, gel electrophoresis, and possibly subcloning, sequencing and sequence analyses to understand the nature of mutations. genotyping by PCR, gel electrophoresis, subcloning, sequence analyses
- Teßmar-Raible: standard molecular and cell biological techniques
- Ries: The project will involve generation of strains labelled with photoconvertible fluorescent proteins and super resolution microscopy. yeast genetics, molecular biology, superresolution microscopy

Information

Aims, contents and method of the course

During this practical course the participants will deal with selected examples of experiments related to the field of molecular microbiology. The methods used are mainly, but not exclusively those acquired during their studies. Design and execution of experiments, documentation and discussion of results have to be summarized in a protocol. Topic: Post-transcriptional regulation of gene expression in bacteria, protein-protein-, RNA-protein-, RNA-RNA-interactions.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Compulsory attendance;
- active participation (discussion contributions, participation, ….): 20%
- results of the performed experiments: 10%
- theoretical understanding and practical knowledge of the methods used: 15%
- documentation and interpretation of the results: 15%
- written final report (bachelor thesis): 40%

Examination topics

Content and scientific background of the performed experiments.

Reading list

Topic-specific literature will be provided.

Association in the course directory

BMB 11

Last modified: Fr 27.06.2025 00:02