Universität Wien FIND
Warning! The directory is not yet complete and will be amended until the beginning of the term.

301641 UE Mass Spectrometry based Proteomics (2017W)

Genetics and biochemistry

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: German, English

Lecturers

Classes

Preparatory meeting: Oct. 4th, 3 pm s.t., SR 3 (6th floor, MFPL)
The participation in the preparatory meeting is mandatory.
Course date: Oct. 23 - Nov. 8 (no course on Oct 26th and Nov. 1st to 3rd)
Course venue: course rooms (6th floor), Dr.-Bohrgasse 9, 1030 Vienna


Information

Aims, contents and method of the course

We offer a practical university course, which provides training in mass spectrometry (MS)-based proteomics. In this laboratory course (limited to 12 students) we will introduce general concepts of MS-based proteomics and discuss criteria that are required to prepare protein samples of sufficient quality and purity for MS analysis. You will perform sample preparation techniques (including affinity purification of proteins, reduction and alkylation of cysteines, digestion with trypsin), followed by RP-HPLC (reversed phase high-pressure liquid chromatography) MS measurement - the operation of the mass spectrometer will be demonstrated. You will also be introduced to MS data analysis using freeware MS data analysis tools such as MaxQuant, SeeMS and Perseus. We will furthermore aim to describe qualitative and quantitative differences between samples by applying various analysis techniques and label free quantification methods.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Examination topics

Reading list


Association in the course directory

MMB I-3, MMB I-4, MMB II-3, MMB II-4, MMB W-2, MGE III-1, MMEI III, MMI III-1

Last modified: Mo 07.09.2020 15:44